Enhanced resistance of yeast mutants deficient in low-affinity iron and zinc transporters to stannous-induced toxicity
| Autor: | Martin Brendel, Jonny F. Dias, Temenouga N. Guecheva, Jacqueline Moraes Cardone, Cristina Pungartnik, M.L. Yoneama, João Antonio Pêgas Henriques, Cassiana Macagnan Viau, Jenifer Saffi |
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| Rok vydání: | 2012 |
| Předmět: |
Environmental Engineering
Health Toxicology and Mutagenesis Saccharomyces cerevisiae Mutant Gene Expression chemistry.chemical_element Zinc Fungal Proteins Superoxide Dismutase-1 Gentamicin protection assay Yeasts Environmental Chemistry chemistry.chemical_classification Reactive oxygen species biology Superoxide Dismutase Public Health Environmental and Occupational Health General Medicine General Chemistry biology.organism_classification Adaptation Physiological Pollution Yeast Biochemistry chemistry Tin Toxicity Environmental Pollutants Carrier Proteins Intracellular |
| Zdroj: | Chemosphere. 86:477-484 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2011.10.007 |
| Popis: | Tin or stannous (Sn 2+ ) compounds are used as catalysts, stabilizers in plastic industries, wood preservatives, agricultural biocides and nuclear medicine. In order to verify the Sn 2+ up-take and toxicity in yeast cells we utilized a multi-elemental analysis known as particle-induced X-ray emission (PIXE) along with cell survival assays and quantitative real-time PCR. The detection of Sn 2+ by PIXE was possible only in yeast cells in stationary phase of growth (STAT cells) that survive at 25 mM Sn 2+ concentration. Yeast cells in exponential phase of growth (LOG cells) tolerate only micro-molar Sn 2+ concentrations that result in intracellular concentration below of the method detection limit. Our PIXE analysis showed that STAT XV185-14c yeast cells demonstrate a significant loss of intracellular elements such as Mg, Zn, S, Fe and an increase in P levels after 1 h exposure to SnCl 2 . The survival assay showed enhanced tolerance of LOG yeast cells lacking the low-affinity iron and zinc transporters to stannous treatment, suggesting the possible involvement in Sn 2+ uptake. Moreover, our qRT-PCR data showed that Sn 2+ treatment could generate reactive oxygen species as it induces activation of many stress-response genes, including SOD1 , YAP1 , and APN1 . |
| Databáze: | OpenAIRE |
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