Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
| Autor: | Levi Dygert, Luc Bertrand, Michal Toborek |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment General Chemical Engineering Ischemia Infarction Fluorescent Antibody Technique General Biochemistry Genetics and Molecular Biology Brain Ischemia 03 medical and health sciences Global population Mice 0302 clinical medicine Medicine Animals Artery occlusion Middle cerebral artery occlusion cardiovascular diseases Stroke General Immunology and Microbiology business.industry General Neuroscience Infarction Middle Cerebral Artery Microsurgery medicine.disease Disease Models Animal 030104 developmental biology Anesthesia Reperfusion Injury Ischemic stroke business 030217 neurology & neurosurgery |
| Zdroj: | Journal of visualized experiments : JoVE. (120) |
| ISSN: | 1940-087X |
| Popis: | Cerebrovascular disease is highly prevalent in the global population and encompasses several types of conditions, including stroke. To study the impact of stroke on tissue injury and to evaluate the effectiveness of therapeutic interventions, several experimental models in a variety of species were developed. They include complete global cerebral ischemia, incomplete global ischemia, focal cerebral ischemia, and multifocal cerebral ischemia. The model described in this protocol is based on the middle cerebral artery occlusion (MCAO) and is related to the focal ischemia category. This technique produces consistent focal ischemia in a strictly defined region of the hemisphere and is less invasive than other methods. The procedure described is performed on mice, given the availability of several genetic variants and the high number of tests standardized for mice to aid in the behavioral and neurodeficit evaluation. |
| Databáze: | OpenAIRE |
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