Evaluating the mechanism underlying antitumor effect of interleukin 27 on B cells of chronic lymphocytic leukemia patients
| Autor: | Mehdi Barati, Parviz Kokhaei, Shahrbano Rostami, Amir Abbas Momtazi-Borojeni, Ehsan Manouchehri‐Doulabi, Somaye Abbaspour, F. Ghahramanfard, Fatemeh Pak, Mohammad Faranoush |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male CD96 Physiology T cell Receptor expression Lymphocyte Chronic lymphocytic leukemia T-Lymphocytes Clinical Biochemistry Apoptosis Lymphocyte Activation Natural killer cell 03 medical and health sciences 0302 clinical medicine medicine Humans Interleukin 27 Aged Cell Proliferation Aged 80 and over B-Lymphocytes Chemistry Interleukins Cell Biology Middle Aged medicine.disease Leukemia Lymphocytic Chronic B-Cell Killer Cells Natural Leukemia 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Leukocytes Mononuclear Female |
| Zdroj: | Journal of cellular physiologyREFERENCES. 235(12) |
| ISSN: | 1097-4652 |
| Popis: | Chronic lymphocyte leukemia (CLL) is a B-cell malignancy resisted to apoptosis. Recently, some studies indicated that cytokines such as interleukin 27 (IL-27) can reduce B-cell proliferation. The aim of this study is to evaluate the mechanism underlying the proapoptotic effect of IL-27 on B cells of patients with CLL in comparison with B cells of normal subjects. The effect of IL-27 on the antitumor activity of natural killer (NK) and T cells was also evaluated. Peripheral blood mononuclear cells (PBMCs) were isolated from 35 patients with CLL and 15 normal subjects. B cells and PBMCs were cocultured with IL-27 and B cells apoptosis to evaluate proliferation. Both messenger RNA and protein expression of IL-27 and IL-27 receptor were determined using flow cytometry and real-time polymerase chain reaction analysis. To evaluate the apoptotic effect of IL-27 on B cells of patients with CLL, Annexin V-FITC and 7-AAD (BioLegend) fluorescent dyes were used. In addition, the IL-27 effect on activation of T cell and NK cell was determined by determining CD96 molecule expression. IL-27 and IL-27 receptor expression in patients with CLL was significantly lower than that of normal subjects (p .05). IL-27 enhanced apoptosis of B cells in patients with CLL (p .05) but this effect was not significantly observed in B cells of normal subjects (p .05). Consequently, IL-27 reduced the proliferation of B cells and enhanced NK cell activity (p .05). IL-27, through inducing apoptosis, can exert an inhibitory effect on cancer B cells of CLL patients with minimal effect on normal B cells. |
| Databáze: | OpenAIRE |
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