Transcriptome analysis of the human T lymphocyte cell line Jurkat and human peripheral blood mononuclear cells exposed to deoxynivalenol (DON): New mechanistic insights
| Autor: | Peter J.M. Hendriksen, Jia Shao, Henk Van Loveren, Ad A. C. M. Peijnenburg, Madhumohan R. Katika |
|---|---|
| Přispěvatelé: | Promovendi ODB, Toxicogenomics, RS: GROW - School for Oncology and Reproduction |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
vomitoxin deoxynivalenol
Time Factors Novel Foods & Agrochains Microarrays T-Lymphocytes RIKILT - Business Unit Veiligheid & Gezondheid Apoptosis er stress Novel Foods & Agroketens Toxicology Jurkat cells Transcriptome oxidative stress Immunotoxicity BU Toxicology Novel Foods & Agrochains activated protein-kinases Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction T cell activation BU Toxicology Human peripheral blood mononuclear cells (PBMCs) NF-kappa B NFAT Endoplasmic Reticulum Stress Up-Regulation Cell biology messenger-rna expression medicine.anatomical_structure BU Toxicologie Novel Foods & Agroketens cytokine production ER stress Signal Transduction BU Toxicologie T cell in-vitro exposure Biology Peripheral blood mononuclear cell Immune system Deoxynivalenol (DON) nf-kappa-b medicine Humans Pharmacology Dose-Response Relationship Drug NFATC Transcription Factors Microarray analysis techniques Gene Expression Profiling stress-induced apoptosis Gene Expression Regulation Cell culture Oxidative stress RIKILT - Business Unit Safety & Health Leukocytes Mononuclear Calcium Trichothecenes trichothecene mycotoxins |
| Zdroj: | Toxicology and Applied Pharmacology 264 (2012) 1 Toxicology and Applied Pharmacology, 264(1), 51-64. Elsevier Science Toxicology and Applied Pharmacology, 264(1), 51-64 |
| ISSN: | 0041-008X |
| Popis: | Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid cells. For this, we exposed the human T-lymphocyte cell line Jurkat and human peripheral blood mononuclear cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat cells were exposed to 0.25 and 0.5 mu M DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-kappa B/TNF-alpha pathways, T cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T cell activation and apoptosis. Induction of T cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, cells were exposed to 2 and 4 mu M DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat cell line were also affected in the PBMCs. (C) 2012 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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