Laser capture microdissection of cells from plant tissues
| Autor: | Timothy Nelson, S. Lorraine Tausta, Teresa Ceserani, Nancy M Kerk, Ian M. Sussex |
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| Rok vydání: | 2003 |
| Předmět: |
Cell type
Tissue Fixation DNA Plant Physiology Plant Science Biology Sensitivity and Specificity chemistry.chemical_compound Plant Cells Gene expression Genetics Microdissection Laser capture microdissection Plant Proteins Histocytological Preparation Techniques Tissue Embedding Gene Expression Profiling Lasers fungi food and beverages RNA Plants Breakthrough Technologies Plant cell Molecular biology Cell biology Gene expression profiling chemistry RNA Plant DNA |
| Zdroj: | Plant physiology. 132(1) |
| ISSN: | 0032-0889 |
| Popis: | Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types. |
| Databáze: | OpenAIRE |
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