The use of Eudragit RS 100/cyclodextrin nanoparticles for the transmucosal administration of glutathione
| Autor: | Giuseppe Trapani, Angela Lopedota, Rosa Curci, Elena Pantucci, Adriana Trapani, Annalisa Cutrignelli, Elisabetta Manuali, Laura Chiarantini |
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| Rok vydání: | 2008 |
| Předmět: |
Stereochemistry
Swine Acrylic Resins Pharmaceutical Science Nanoparticle Inclusion compound Cell Line Crystallinity chemistry.chemical_compound Mice X-Ray Diffraction Transmucosal administration GSH Animals Humans Horses Microparticle Cytotoxicity in vitro chemistry.chemical_classification Cyclodextrins Mucous Membrane Cyclodextrin Eudragit RS 100 Nanoparticles Rana esculenta General Medicine Glutathione Solvent HaCaT chemistry Biotechnology Nuclear chemistry |
| Zdroj: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 72(3) |
| ISSN: | 1873-3441 |
| Popis: | The aim of this work was to develop and characterize new nanoparticle systems based on Eudragit RS 100 and cyclodextrins (CDs) for the transmucosal administration of glutathione (GSH). For this purpose, nanoparticles (NPs) with the mucoadhesive properties of Eudragit RS 100 and the penetration enhancing and peptide protective properties of CDs were prepared and evaluated. The quasi-emulsion solvent diffusion technique was used to prepare the NPs with natural and chemically modified (HP-beta-CD and Me-beta-CD) CDs. The NPs prepared showed homogeneous size distribution, mean diameters between 99 and 156nm, a positive net charge and spherical morphology. Solid state FT-IR, thermal analysis (DSC), and X-ray diffraction studies suggest that the nanoencapsulation process produces a marked decrease in crystallinity of GSH. The encapsulation efficiency of the peptide was found to be between 14.8% and 24%. The results indicate that mean diameters, surface charges and drug-loaded NPs were not markedly affected by the CD, whereas the presence of the latter influences drug release and to some extent peptide stability and absorption. Finally, it has been shown that CD/Eudragit RS 100 NPs may be used for transmucosal absorption of GSH without any cytotoxicity using the epithelial human HaCaT and murine monocyte macrophage RAW264.7 cell lines. |
| Databáze: | OpenAIRE |
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