In-vitro effect of Peganum harmala total alkaloids on spermatozoa quality and oxidative stress of epididymal ram semen
| Autor: | Mohamed Moussaoui, Hanane Derbak, Amine Benberkane, Abdelhanine Ayad |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
General Veterinary biology Chemistry Alkaloid alkaloids epididymal sperm motility peganum harmala ram semen membrane integrity lipid peroxidation Obstetrics and Gynecology Semen Plant Science Peganum biology.organism_classification medicine.disease_cause Sperm Andrology Lipid peroxidation chemistry.chemical_compound Reproductive Medicine Peganum harmala medicine Medicine Animal Science and Zoology Sperm motility Oxidative stress |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 10, Iss 5, Pp 232-238 (2021) |
| ISSN: | 2305-0519 2305-0500 |
| Popis: | Objective: To determine the in-vitro effect of the total alkaloid extract of Peganum (P.) harmala seeds on ram epididymal sperm. Methods: Semen was divided into six groups according to the following concentrations of the P. harmala total alkaloids: 1, 5, 10, 50, and 100 μg/mL, and the control group. The samples were incubated at ambient temperature (21 °C-24 °C) for 24 h, and analyzed in terms of motility, membrane integrity, and oxidative status. Results: The sperm kinematic parameters, i.e. straight-line velocity, curvilinear velocity, average path velocity, were significantly higher when treated with P. harmala at concentrations ranging from 1 to 10 μg/mL compared to the control group (P0.05). Conclusions: Low concentrations (1-10 μg/mL) of P. harmala extracts stimulate sperm motility, preserve membrane integrity and protect ram spermatozoa from lipid peroxidation. |
| Databáze: | OpenAIRE |
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