Drosophila Models Rediscovered with Super-Resolution Microscopy
Autor: | József Mihály, Csaba Kozma, Szilárd Szikora, Péter Görög |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Sarcomeres
Molecular complexity Computer science QH301-705.5 ved/biology.organism_classification_rank.species Review active zone Computational biology nanoscopy Models Biological 03 medical and health sciences 0302 clinical medicine super-resolution microscopy Animals Biology (General) Model organism SMLM Drosophila 030304 developmental biology Centrosome 0303 health sciences biology Super-resolution microscopy ved/biology STED microscopy General Medicine SIM biology.organism_classification Single Molecule Imaging STED Microscopy Fluorescence sarcomere 030217 neurology & neurosurgery |
Zdroj: | Cells, Vol 10, Iss 1924, p 1924 (2021) Cells |
ISSN: | 2073-4409 |
Popis: | With the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjects because combining the strength of sophisticated genetics, biochemistry and electrophysiology with the unparalleled resolution provided by super-resolution imaging appears as one of the most efficient approaches to understanding the basic cell biological questions and the molecular complexity of life. Here, we summarize the major nanoscopic techniques and illustrate how these approaches were used in Drosophila model systems to revisit a series of well-known cell biological phenomena. These investigations clearly demonstrate that instead of simply achieving an improvement in image quality, nanoscopy goes far beyond with its immense potential to discover novel structural and mechanistic aspects. With the examples of synaptic active zones, centrosomes and sarcomeres, we will explain the instrumental role of super-resolution imaging pioneered in Drosophila in understanding fundamental subcellular constituents. |
Databáze: | OpenAIRE |
Externí odkaz: |