The TZM-bl Reporter Cell Line Expresses Kynureninase That Can Neutralize 2F5-like Antibodies in the HIV-1 Neutralization Assay
Autor: | Sylvie Lagaye, Alexey Morozov, Morozov Vladimir |
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Přispěvatelé: | HAL-SU, Gestionnaire, University of Maryland School of Medicine, University of Maryland System, Robert Koch Institute [Berlin] (RKI), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Engelhardt Institute of Molecular Biology (ISTC), Russian Academy of Sciences [Moscow] (RAS) |
Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
QH301-705.5
Hydrolases TZM-bl cell line HIV Antibodies sera Catalysis Article Inorganic Chemistry Epitopes Jurkat Cells Protein Domains Neutralization Tests neutralization assay Chlorocebus aethiops expression Animals Humans Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Vero Cells Spectroscopy [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology Organic Chemistry virus diseases Antibodies Monoclonal General Medicine Hep G2 Cells Antibodies Neutralizing HIV-1 mAb 2F5 mAb 4E10 kynureninase HIV Envelope Protein gp41 Computer Science Applications Chemistry HEK293 Cells Broadly Neutralizing Antibodies [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology HeLa Cells |
Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2022, 23 (2), pp.641. ⟨10.3390/ijms23020641⟩ International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 641 International Journal of Molecular Sciences, Vol 23, Iss 641, p 641 (2022) |
ISSN: | 1661-6596 1422-0067 |
Popis: | Induction of broadly neutralizing antibodies targeting ectodomain of the transmembrane (TM) glycoprotein gp41 HIV-1 provides a basis for the development of a universal anti-viral vaccine. The HeLa cell-derived TZM-bl reporter cell line is widely used for the estimation of lentiviruses neutralization by immune sera. The cell line is highly permissive to infection by most strains of HIV, SIV, and SHIV. Here we demonstrated that TZM-bl cells express a 48 kDa non-glycosylated protein (p48) recognized by broadly neutralizing monoclonal antibody (mAb) 2F5 targeting the ELDKWA (aa 669–674) epitope of gp41TM of HIV-1. A significant amount of p48 was found in the cell supernatant. The protein was identified as human kynureninase (KYNU), which has the ELDKWA epitope. The protein is further called “p48 KYNU”. The HIV-1 neutralization by mAb 2F5 and 4E10 in the presence of p48KYNU was tested on Jurkat and TZM-bl cells. It was demonstrated that p48KYNU reduces neutralization by 2F5-like antibodies, but it has almost no effect on mAb 4E10. Therefore, p48KYNU can attenuate HIV-1 neutralization by 2F5-like antibodies and hence create false-negative results. Thus, previously tested immune sera that recognized the ELDKWA-epitope and demonstrated a “weak neutralization” of HIV-1 in TZM-bl assay should be reevaluated. |
Databáze: | OpenAIRE |
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