The Left-Right Pitx2 Pathway Drives Organ-Specific Arterial and Lymphatic Development in the Intestine
Autor: | David W. Gludish, Abigail R. Shilvock, David Huss, Ian C. Welsh, Rusty Lansford, Natasza A. Kurpios, Aravind Sivakumar, Drew M. Noden, Aparna Mahadevan |
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Jazyk: | angličtina |
Předmět: |
Receptors
CXCR4 Dorsal mesentery Green Fluorescent Proteins Morphogenesis Mice Transgenic Biology Angioblast Quail General Biochemistry Genetics and Molecular Biology Article Lymphatic System Mice Organ specific Animals Mesentery Lymphangiogenesis Molecular Biology Body Patterning Lymphatic Vessels Oligonucleotide Array Sequence Analysis Homeodomain Proteins PITX2 Gene Expression Regulation Developmental Cell Biology Anatomy Arteries Chemokine CXCL12 Cell biology Intestines Lymphatic system Microscopy Fluorescence Arteriogenesis Chickens Transcription Factors Developmental Biology |
Zdroj: | Developmental Cell. (6):690-706 |
ISSN: | 1534-5807 |
DOI: | 10.1016/j.devcel.2014.11.002 |
Popis: | Summary The dorsal mesentery (DM) is the major conduit for blood and lymphatic vessels in the gut. The mechanisms underlying their morphogenesis are challenging to study and remain unknown. Here we show that arteriogenesis in the DM begins during gut rotation and proceeds strictly on the left side, dependent on the Pitx2 target gene Cxcl12 . Although competent Cxcr4 -positive angioblasts are present on the right, they fail to form vessels and progressively emigrate. Surprisingly, gut lymphatics also initiate in the left DM and arise only after—and dependent on—arteriogenesis, implicating arteries as drivers of gut lymphangiogenesis. Our data begin to unravel the origin of two distinct vascular systems and demonstrate how early left-right molecular asymmetries are translated into organ-specific vascular patterns. We propose a dual origin of gut lymphangiogenesis in which prior arterial growth is required to initiate local lymphatics that only subsequently connect to the vascular system. |
Databáze: | OpenAIRE |
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