Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: An in vitro and in vivo study
| Autor: | Bernard Gallez, Bernard Masereel, Olivier Feron, Linda Karmani, Vanessa Valembois, Riccardo Marega, Lionel Flamant, Davide Bonifazi, Thierry Vander Borght, Stéphane Lucas, Praveen Ganesh Nageswaran, Carine Michiels |
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| Přispěvatelé: | R., Marega, L., Karmani, L., Flamant, P., Nageswaran, V., Valemboi, B., Masereel, O., Feron, T., Borght, S., Luca, C., Michiel, B., Gallez, Bonifazi, Davide |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Biodistribution
Cancer cells Materials science medicine.drug_class Allyl amine Biodistributions Cancer cells Cancer-targeting Cell lines Epidermal growth factor receptors Functionalizations Gold Nanoparticles In-vitro In-vivo Western blots Cell culture Metal nanoparticles Monoclonal antibodies Plasmas Vapors Gold Western blots Metal nanoparticles Functionalizations Cell culture Metal nanoparticles Monoclonal antibodies Plasmas Vapor Biodistributions Monoclonal antibody Allylamine In-vitro chemistry.chemical_compound In-vivo In vivo Cancer-targeting Epidermal growth factor receptors Materials Chemistry medicine Gold Nanoparticles Epidermal growth factor receptor biology Allyl amine General Chemistry Molecular biology Allyl amine Biodistributions Cancer cells Cancer-targeting Cell lines Epidermal growth factor receptors Functionalizations Gold Nanoparticles In-vitro In-vivo Western blot chemistry Plasmas Colloidal gold Vapors biology.protein Cell lines Monoclonal antibodies Cell culture Antibody Nanoconjugates |
| Zdroj: | Marega, R, Karmani, L, Flamant, L, Nageswaran, P G, Valembois, V, Masereel, B, Feron, O, Vander Borght, T, Lucas, S, Michiels, C, Gallez, B & Bonifazi, D 2012, ' Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study ', Journal of Materials Chemistry, vol. 22, no. 39, pp. 21305-21312 . https://doi.org/10.1039/c2jm33482h |
| DOI: | 10.1039/c2jm33482h |
| Popis: | Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg -1 and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is © 2012 The Royal Society of Chemistry. |
| Databáze: | OpenAIRE |
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