Real-time PCR Biochip for On-Site Detection of Coxiella Burnetii in Ticks
| Autor: | Youngmin Yun, Ji-Yeon Lim, Soon-Seek Yoon, Mi-Sun Yoo, Bo-Ram Yun, Subin Min, A-Tai Truong, Jong-Taek Kim, Yun Sang Cho |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Ixodidae 030231 tropical medicine Q fever Infectious and parasitic diseases RC109-216 Biology Tick Real-Time Polymerase Chain Reaction Haemaphysalis longicornis Sensitivity and Specificity law.invention 03 medical and health sciences 0302 clinical medicine law medicine Animals Humans Asian longhorned tick Polymerase chain reaction Tick-borne disease Ultra-rapid real-time PCR Research Zoonosis Arthropod Vectors medicine.disease biology.organism_classification Coxiella burnetii bacterial infections and mycoses Virology 030104 developmental biology Infectious Diseases Parasitology Genes Bacterial Tick-Borne Diseases bacteria Q Fever |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 14, Iss 1, Pp 1-8 (2021) |
| Popis: | Background Q fever, a zoonosis caused by Coxiella burnetii, has adverse effects on public health. Ticks are vectors of C. burnetii and they contribute to the transmission of the pathogen. A tool for rapid, sensitive, and accurate detection of C. burnetii from ticks is important for the prevention of Q fever. Methods Ultra-rapid real-time PCR (UR-qPCR) as a chip-based real-time PCR system was developed for the detection of C. burnetii from ticks. The UR-qPCR system was established and evaluated for the rapidity, sensitivity, and specificity of C. burnetii detection. Results C. burnetii was detected using UR-qPCR from 5644 larval, nymphal, and adult ticks from 408 pools collected from livestock and epidemiologically linked environments in two provinces, Gangwon and Jeju, in Korea. Ticks from three species were identified; Haemaphysalis longicornis accounted for the highest number, present in 333 of 408 pools (81.62%), followed by Haemaphysalis flava in 62 pools (15.19%) and Ixodes nipponensis in 13 pools (3.19%). The rapidity and sensitivity of PCR detection was demonstrated with the sufficient amplification and detection of approximately 56 copies of C. burnetii DNA with only 20 min of PCR amplification. The kappa value for the diagnostic agreement between UR-qPCR and stationary qPCR was in perfect agreement (κ = 1). PCR detection and sequencing indicated that C. burnetii was present in 5 of the 408 pools (1.23%), in which four pools contained H. longicornis and one pool contained H. flava. The infection rates of C. burnetii in the tick pools collected from Gangwon and Jeju Provinces were 1.70% and 0.58%, respectively. Phylogenetic analysis indicated a close relationship between the detected C. burnetii and those originating from goats, humans, and ticks in different countries, such as the USA, France, Germany, and Serbia. Conclusions The methods described in this study could be important for the prevention and control of Q fever in the two provinces. The UR-qPCR, with its features of mobility, sensitivity, and rapidity, is helpful for constructing early alert systems in the field for C. burnetii in ticks and could help alleviate the transmission of and economic damage due to Q fever. Graphical Abstract |
| Databáze: | OpenAIRE |
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