Phosphodiesterase type 4 that regulates cAMP level in cortical neurons shows high sensitivity to rolipram
| Autor: | Jun Baba, Aiko Sawa, Nobuyuki Yamashita, Miki Yamauchi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Phosphodiesterase Inhibitors Phosphodiesterase 3 Biology Muscle Smooth Vascular Internal medicine Cyclic AMP medicine Animals Humans Rats Wistar Rolipram Cerebral Cortex Neurons Pharmacology Phosphoric Diester Hydrolases Isoproterenol Phosphodiesterase Adrenergic beta-Agonists Pyrrolidinones Rats medicine.anatomical_structure Endocrinology Guanylate Cyclase Cerebral cortex Astrocytes PDE10A Neuron Intracellular Adenylyl Cyclases medicine.drug |
| Zdroj: | European Journal of Pharmacology. 337:95-102 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/s0014-2999(97)01285-5 |
| Popis: | To characterize the role of phosphodiesterase type 4 (a cAMP-specific and rolipram-sensitive phosphodiesterase) among phosphodiesterases in the regulation of the intracellular cAMP level in cortical neurons, we investigated the effects of phosphodiesterase inhibitors on the intracellular cAMP levels in primary cultured rat cortical neurons. Selective inhibitors of phosphodiesterase type 4 and type 2 significantly enhanced beta-adrenoceptor-mediated cAMP increase. Selective inhibitors of phosphodiesterase type 1, type 3 and type 5/6 had no effect on the cAMP level. Rolipram enhanced the beta-adrenoceptor-mediated cAMP increase in cortical neurons, astrocytes and vascular smooth muscle cells at different minimum effective concentrations (10, 100 and 1000 nM, respectively). These findings indicate that phosphodiesterase type 4, showing a high-sensitivity to rolipram, plays a major role in regulating cAMP in the cortical neurons, and that rolipram at low doses enhances the intracellular cAMP increase in the cortical neurons selectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect