Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows
| Autor: | Thomas H. Herdt, Jaimie M. Strickland, Doug Lyman, John P. Buchweitz, Lorraine M. Sordillo |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Article Subject 040301 veterinary sciences Sorbitol dehydrogenase medicine.drug_class medicine.disease_cause 0403 veterinary science Liver disease Fibrosis Internal medicine medicine lcsh:Veterinary medicine General Veterinary Bile acid Chemistry Glutamate dehydrogenase 0402 animal and dairy science 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science Staining Endocrinology medicine.anatomical_structure Hepatocyte lcsh:SF600-1100 Oxidative stress Research Article |
| Zdroj: | Veterinary Medicine International Veterinary Medicine International, Vol 2019 (2019) |
| ISSN: | 2042-0048 2090-8113 |
| Popis: | Concerns regarding excessive hepatic copper concentrations in dairy cows have increased. The objective of this study was to determine the association of hepatic copper concentrations with evidence of liver disease. Blood and liver samples were collected at the time of slaughter in cull dairy cows (n=100). Liver samples were analyzed for copper using inductively coupled plasma mass spectrometry and crude fat using liquid-liquid extraction and gravimetry. Serum samples were analyzed for glutamate dehydrogenase,γ-glutamyltransferase, sorbitol dehydrogenase, aspartate aminotransferase activities, and bile acid concentrations. Liver samples were examined histologically for inflammation, fibrosis, and rhodanine staining. Animals were stratified by hepatic copper concentration and samples in the highest and lowest quintiles (Q5 and Q1) were evaluated for oxidative stress. Systemic indices of oxidative stress included serum reactive oxygen and nitrogen species (RONS) and total antioxidant potential (AOP). Tissue-level oxidative stress was assessed by immunohistochemistry using 4-hydroxynonenal (4HNE) and 3-nitrotyrosine (3NIT) stains to score the relative abundance and distribution of oxidized lipid and protein products, respectively. Mean hepatic copper concentration was 496.83μg/g and median 469.72μg/g and ranged from 70.56 to 1264.27μg/g dry tissue. No association was found between hepatic copper concentrations and clinicopathological or histological evidence of hepatic damage or dysfunction. There was a significant increase in the amount of IHC staining of 4HNE and 3NIT in Q5 compared with Q1. Moreover, the IHC staining mirrored the distribution of the copper-specific stain rhodanine. These results demonstrate that cows with elevated hepatic copper concentrations had no evidence of active liver disease but had increased hepatic oxidative stress. |
| Databáze: | OpenAIRE |
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