Improved production of jiangxienone in submerged fermentation of Cordyceps jiangxiensis under nitrogen deficiency
Autor: | Jian-Hui Xiao, Jun-Wei Xu, Hui-Ping Wang, Lu-Xi Jiang, Li-Liang Han |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Nitrogen Bioengineering 01 natural sciences Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene Expression Regulation Fungal 010608 biotechnology Cytotoxicity Regulator gene Cordyceps biology Cyclohexanones Nitrogen deficiency General Medicine biology.organism_classification Glutamine 030104 developmental biology chemistry Biochemistry Indans Cancer cell Mevalonate pathway Biotechnology |
Zdroj: | Bioprocess and Biosystems Engineering. 41:1417-1423 |
ISSN: | 1615-7605 1615-7591 |
Popis: | Jiangxienone produced by Cordyceps jiangxiensis exhibits significant cytotoxicity and good selectivity against various human cancer cells, especially gastric cancer cells. In this work, the effect of nitrogen deficiency on the accumulation of jiangxienone and the transcription levels of jiangxienone biosynthesis genes was studied in submerged fermentation of C. jiangxiensis. Results showed that accumulation of jiangxienone was improved under nitrogen deficiency condition. A maximal jiangxienone content of 3.2 µg/g cell dry weight was reached at 5 mM glutamine, and it was about 8.9-fold higher than that obtained at 60 mM glutamine (control). The transcription levels of the biosynthetic pathway genes hmgr and sqs and the nitrogen regulatory gene areA were upregulated by 7-, 14-, and 28-fold, respectively, in culture with 5 mM glutamine compared to the control. It was hypothesized that the jiangxienone biosynthesis may involve the mevalonate pathway in C. jiangxiensis. Taken together, our study indicated that nitrogen deficiency is an efficient strategy for enhancing jiangxienone accumulation in submerged fermentation of C. jiangxiensis, which is useful for further understanding the regulation of jiangxienone biosynthesis. |
Databáze: | OpenAIRE |
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