Insulin Modulates STAT3 Protein Activation and Gene Transcription in Hepatic Cells
| Autor: | Susana P. Campos, Yanping Wang, Heinz Baumann |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
STAT3 Transcription Factor
Transcription Genetic Insulin Receptor Substrate Proteins medicine.medical_treatment Biology Biochemistry Antigens CD Insulin receptor substrate Tumor Cells Cultured medicine Animals Insulin RNA Messenger STAT3 Molecular Biology Dose-Response Relationship Drug Interleukin-6 GRB10 Intracellular Signaling Peptides and Proteins Receptors Interleukin Cell Biology Phosphoproteins Receptors Interleukin-6 Molecular biology IRS2 Rats DNA-Binding Proteins Kinetics Insulin receptor Liver Trans-Activators biology.protein RFX6 Signal Transduction |
| Zdroj: | Journal of Biological Chemistry. 271:24418-24424 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.40.24418 |
| Popis: | Treatment of rat hepatoma cells with insulin attenuated the interleukin 6 (IL-6) stimulation of acute phase plasma protein genes. To identify the potential mechanism of this action, the influence of insulin on IL-6 signal transduction was determined. An insulin dose-dependent reduction in signal transducer and activator of transcription 3 (STAT3) gene transcription, mRNA accumulation, protein concentration, and IL-6-inducible DNA binding activity was detected. A reduction in the IL-6-activated sis-inducible element binding of STAT3 was observed within 4 h of insulin treatment, whereas a maximal 3-4-fold lower STAT protein concentration was measured after 8-24 h of insulin treatment. Insulin mediated a similar magnitude reduction in the amount of mRNA encoding the IL-6 receptor alpha subunit and IL-6 binding activity. These effects of insulin appear to contribute to the strongly suppressed transcriptional induction of the IL-6-responsive acute phase plasma protein genes. |
| Databáze: | OpenAIRE |
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