Multi LC-MS/MS and LC-HRMS Methods for Determination of 24 Mycotoxins including Major Phase I and II Biomarker Metabolites in Biological Matrices from Pigs and Broiler Chickens
| Autor: | Mathias Devreese, Ben Letor, Marianne Lauwers, Siska Croubels, Siegrid De Baere, Michael Rychlik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Ochratoxin A
pig Aflatoxin LC-HRMS Swine Health Toxicology and Mutagenesis MASKED MYCOTOXIN Ethyl acetate lcsh:Medicine Toxicology 01 natural sciences chemistry.chemical_compound Feces ZEARALENONE Tandem Mass Spectrometry ddc:630 Sample preparation Zearalenone PLASMA biology Chemistry digestive oral and skin physiology COMPARATIVE TOXICOKINETICS food and beverages 04 agricultural and veterinary sciences 040401 food science ddc multi-mycotoxin Biological Monitoring Fusarium animal structures T-2 TOXIN Article 0404 agricultural biotechnology QUANTITATIVE-DETERMINATION ENNIATIN B1 Animals Veterinary Sciences LC-MS/MS Mycotoxin Chromatography 010401 analytical chemistry Extraction (chemistry) lcsh:R Biology and Life Sciences Mycotoxins biology.organism_classification 0104 chemical sciences broiler chicken exposure MASS-SPECTROMETRY METHOD ORAL BIOAVAILABILITY DEOXYNIVALENOL DON Chickens Biomarkers Chromatography Liquid |
| Zdroj: | Toxins Toxins, Vol 11, Iss 3, p 171 (2019) Volume 11 Issue 3 TOXINS |
| ISSN: | 2072-6651 |
| Popis: | A reliable and practical multi-method was developed for the quantification of mycotoxins in plasma, urine, and feces of pigs, and plasma and excreta of broiler chickens using liquid chromatography&ndash tandem mass spectrometry. The targeted mycotoxins belong to the regulated groups, i.e., aflatoxins, ochratoxin A and Fusarium mycotoxins, and to two groups of emerging mycotoxins, i.e., Alternaria mycotoxins and enniatins. In addition, the developed method was transferred to a LC-high resolution mass spectrometry instrument to qualitatively determine phase I and II metabolites, for which analytical standards are not always commercially available. Sample preparation of plasma was simple and generic and was accomplished by precipitation of proteins alone (pig) or in combination with removal of phospholipids (chicken). A more intensive sample clean-up of the other matrices was needed and consisted of a pH-dependent liquid&ndash liquid extraction (LLE) using ethyl acetate (pig urine), methanol/ethyl acetate/formic acid (75/24/1, v/v/v) (pig feces) or acetonitrile (chicken excreta). For the extraction of pig feces, additionally a combination of LLE using acetone and filtration of the supernatant on a HybridSPE-phospholipid cartridge was applied. The LC-MS/MS method was in-house validated according to guidelines defined by the European and international community. Finally, the multi-methods were successfully applied in a specific toxicokinetic study and a screening study to monitor the exposure of individual animals. |
| Databáze: | OpenAIRE |
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