C. elegans Dynamin-Related Protein DRP-1 Controls Severing of the Mitochondrial Outer Membrane

Autor: Alexander M. van der Bliek, Daniel A. Rube, Mauro D. Zappaterra, Arnaud M. Labrousse
Rok vydání: 1999
Předmět:
Dynamins
inorganic chemicals
Mitochondrial intermembrane space
Recombinant Fusion Proteins
Translocase of the outer membrane
Molecular Sequence Data
Vesicular Transport Proteins
Gene Expression
macromolecular substances
Biology
environment and public health
Mitochondrial apoptosis-induced channel
Mitochondrial membrane transport protein
Oogenesis
Consensus Sequence
Animals
Drosophila Proteins
Humans
Cell Lineage
RNA
Messenger
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Inner mitochondrial membrane
Molecular Biology
Sequence Homology
Amino Acid
Gene Expression Profiling
Muscles
Neuropeptides
Membrane Proteins
Intracellular Membranes
Cell Biology
Mitochondrial carrier
Mitochondria
Cell biology
Mutation
Translocase of the inner membrane
biology.protein
Tetradecanoylphorbol Acetate
Female
Genes
Lethal
ATP–ADP translocase
Cell Division
Zdroj: Molecular Cell. 4:815-826
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(00)80391-3
Popis: Little is known about the mechanism of mitochondrial division. We show here that mitochondria are disrupted by mutations in a C. elegans dynamin-related protein (DRP-1). Mutant DRP-1 causes the mitochondrial matrix to retract into large blebs that are both surrounded and connected by tubules of outer membrane. This indicates that scission of the mitochondrial outer membrane is inhibited, while scission of the inner membrane still occurs. Overexpressed wild-type DRP-1 causes mitochondria to become excessively fragmented, consistent with an active role in mitochondrial scission. DRP-1 fused to GFP is observed in spots on mitochondria where scission eventually occurs. These data indicate that wild-type DRP-1 contributes to the final stages of mitochondrial division by controlling scission of the mitochondrial outer membrane.
Databáze: OpenAIRE
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