Providence virus: An animal virus that replicates in plants or a plant virus that infects and replicates in animal cells?
| Autor: | Rosemary A. Dorrington, Rachel Anne Jarvie, Mart-Mari de Bruyn, Dylan Waterworth, Meesbah Jiwaji, Janet Awino Awando, Gwynneth F. Matcher, Holisha Moodley |
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| Rok vydání: | 2019 |
| Předmět: |
Leaves
viruses Insect Plant Science Pathology and Laboratory Medicine RNA Virus Infections Medicine and Health Sciences media_common 0303 health sciences Multidisciplinary biology Plant Anatomy food and beverages Eukaryota Animal virus Plants Lepidoptera Insects Medical Microbiology Viral Pathogens Vertebrates Viruses MCF-7 Cells Medicine Pathogens Research Article Arthropoda Science media_common.quotation_subject Plant Pathogens Microbiology Deep sequencing Plant Viral Pathogens 03 medical and health sciences Plant virus Plant Cells Virology Animals Humans RNA Viruses Grasses Insect virus Microbial Pathogens 030304 developmental biology 030306 microbiology Vigna fungi Organisms Biology and Life Sciences Zea RNA virus Plant Pathology biology.organism_classification Invertebrates Viral Replication Viral replication Cell culture HeLa Cells |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 6, p e0217494 (2019) |
| ISSN: | 1932-6203 |
| Popis: | IntroductionEmerging viral diseases, most of which are zoonotic, pose a significant threat to global health. There is a critical need to identify potential new viral pathogens and the challenge is to identify the reservoirs from which these viruses might emerge. Deep sequencing of invertebrate transcriptomes has revealed a plethora of viruses, many of which represent novel lineages representing both plant and animal viruses and little is known about the potential threat that these viruses pose.MethodsProvidence virus, an insect virus, was used to establish a productive infection in Vigna unguiculata (cowpea) plants. Providence virus particles purified from these cowpea plants were used to infect two mammalian cell lines.FindingsHere, we present evidence that Providence virus, a non-enveloped insect RNA virus, isolated from a lepidopteran midgut cell line can establish a productive infection in plants as well as in animal cells. The observation that Providence virus can readily infect both plants and mammalian cell culture lines demonstrates the ability of an insect RNA virus to establish productive infections across two kingdoms, in plants and invertebrate and vertebrate animal cell lines.ConclusionsThe study highlights the potential of phytophagous insects as reservoirs for viral re-assortment and that plants should be considered as reservoirs for emerging viruses that may be potentially pathogenic to humans. |
| Databáze: | OpenAIRE |
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