11, 15, 19-Trihydroxy-9-ketoprost-13-enoic acid and 11, 15, 19-trihydroxy-9-ketoprosta-5, 13-dienoic acid in human seminal fluid
| Autor: | D M Desiderio, B S Middleditch, M A Schexnayder, H T Jonsson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1976 |
| Předmět: |
Chromatography
Cell Biology QD415-436 Biochemistry chemistry.chemical_compound Endocrinology trimethylsilyl derivatives chemistry Isolation techniques medicine O-methyl oxime derivatives Prostaglandins gas-liquid chromatography lipids (amino acids peptides and proteins) Prostaglandin E2 O-ethyl oxime derivatives Prostaglandin E1 gas-liquid chromatography-mass spectrometry Incubation medicine.drug |
| Zdroj: | Journal of Lipid Research, Vol 17, Iss 1, Pp 1-6 (1976) |
| ISSN: | 0022-2275 |
| Popis: | Two novel prostaglandins (PG) have been found in human seminal fluid which had been frozen immediately after collection. They were characterized by combined gas-liquid chromatography-mass spectrometry of various derivatives as 19-hydroxy prostaglandin E1 (11, 15, 19-trihydroxy-9-ketoprost-13-enoic acid) and 19-hydroxy prostaglandin E2 (11, 15, 19-trihydroxy-9-ketoprosta-5, 13-dienoic acid). They were present in three to five times the quantity of prostaglandins E1 and E2. Incubation of seminal fluid for 3 hr at 25 degrees C reduced levels of 190H-PGEs2.5-fold and PGE22-fold, while increasing levels of PGAs and PGBs 2-fold. No 190H PGA or 190H PGB was detected in extracts of unincubated fluid. The PGAs, PGBs and their 19-hydroxy analogs are probably artifacts arising metabolically or as a result of classical isolation techniques. |
| Databáze: | OpenAIRE |
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