Clinical and virological findings in patients with Usutu virus infection, northern Italy, 2018
| Autor: | Marco Zoccarato, Luisa Barzon, Gioia Capelli, Monia Pacenti, Carlo Giovanni Doroldi, Francesco Bigolin, Lucia Nardetto, Elisa Franchin, Silvana Pagni, Alessandro Sinigaglia, Fabrizio Montarsi, Maria Elena De Rui, Elektra Peta, Luca Santelli, Silvia Riccetti, Adelaide Milani, Thomas Martello |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Genotyping Techniques Epidemiology viruses encephalitis 030106 microbiology Enzyme-Linked Immunosorbent Assay Antibodies Viral Arbovirus Neutralization Flavivirus Infections Serology Diagnosis Differential 03 medical and health sciences Virology medicine West Nile Virus Animals Humans Usutu virus Phylogeny fever Surveillance Whole Genome Sequencing medicine.diagnostic_test biology Flavivirus Public Health Environmental and Occupational Health virus diseases Nucleic acid test biology.organism_classification medicine.disease Antibodies Neutralizing Culicidae 030104 developmental biology Italy Immunoglobulin M Immunoglobulin G Population Surveillance biology.protein human infection Antibody Sentinel Surveillance West Nile Fever Encephalitis |
| Zdroj: | Eurosurveillance |
| ISSN: | 1560-7917 |
| DOI: | 10.2807/1560-7917.es.2019.24.47.1900180 |
| Popis: | Background Usutu virus (USUV) is a mosquito-borne flavivirus, which shares its transmission cycle with the phylogenetically related West Nile virus (WNV). USUV circulates in several European countries and its activity has increased over the last 5 years. Aim To describe human cases of USUV infection identified by surveillance for WNV and USUV infection in the Veneto Region of northern Italy in 2018. Methods From 1 June to 30 November 2018, all cases of suspected autochthonous arbovirus infection and blood donors who had a reactive WNV nucleic acid test were investigated for both WNV and USUV infection by in-house molecular methods. Anti-WNV and anti-USUV IgM and IgG antibodies were detected by ELISA and in-house immunofluorescence assay, respectively; positive serum samples were further tested by WNV and USUV neutralisation assays run in parallel. Results Eight cases of USUV infection (one with neuroinvasive disease, six with fever and one viraemic blood donor who developed arthralgia and myalgia) and 427 cases of WNV infection were identified. A remarkable finding of this study was the persistence of USUV RNA in the blood and urine of three patients during follow-up. USUV genome sequences from two patients shared over 99% nt identity with USUV sequences detected in mosquito pools from the same area and clustered within lineage Europe 2. Conclusions Clinical presentation and laboratory findings in patients with USUV infection were similar to those found in patients with WNV infection. Cross-reactivity of serology and molecular tests challenged the differential diagnosis. |
| Databáze: | OpenAIRE |
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