Inactivation ofPseudomonas aeruginosabiofilm by dense phase carbon dioxide
| Autor: | Jae Eun Kim, Jeyong Yoon, Youn-Woo Lee, Jinseong Jeong, Sungmin Mun |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Disinfection methods
Time Factors Supercritical carbon dioxide Pseudomonas aeruginosa Microorganism Biofilm Carbon Dioxide Aquatic Science medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Disinfection chemistry.chemical_compound chemistry Biofilms Phase (matter) Carbon dioxide Wettability medicine Confocal laser scanning microscopy Glass Food science Water Science and Technology |
| Zdroj: | Biofouling. 25:473-479 |
| ISSN: | 1029-2454 0892-7014 |
| DOI: | 10.1080/08927010902874876 |
| Popis: | Dense phase carbon dioxide (DPCD) is one of the most promising techniques available to control microorganisms as a non-thermal disinfection method. However, no study on the efficiency of biofilm disinfection using DPCD has been reported. The efficiency of DPCD in inactivating Pseudomonas aeruginosa biofilm, which is known to have high antimicrobial resistance, was thus investigated. P. aeruginosa biofilm, which was not immersed in water but was completely wet, was found to be more effectively inactivated by DPCD treatment, achieving a 6-log reduction within 7 min. The inactivation efficiency increased modestly with increasing pressure and temperature. This study also reports that the water-unimmersed condition is one of the most important operating parameters in achieving efficient biofilm control by DPCD treatment. In addition, observations by confocal laser scanning microscopy revealed that DPCD treatment not only inactivated biofilm cells on the glass coupons but also caused detachment of the biofilm following weakening of its structure as a result of the DPCD treatment; this is an added benefit of DPCD treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|