Transforming growth factor β1influences lysyl hydroxylation of collagen I and reduces steady-state levels of lysyl hydroxylase mRNA in human osteoblast-Iike celIs
| Autor: | Peter K. Müller, U. Seitzer, Y. Acil, B. Bätge |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Glycosylation medicine.medical_treatment Lysyl hydroxylase Clinical Biochemistry Lysine Lysyl oxidase macromolecular substances Hydroxylation Hydroxylysine Biochemistry chemistry.chemical_compound Transforming Growth Factor beta medicine Humans RNA Messenger Cloning Molecular Osteoblasts integumentary system biology Procollagen-Lysine 2-Oxoglutarate 5-Dioxygenase Growth factor Osteoblast General Medicine Transforming growth factor beta Osteogenesis Imperfecta Blotting Northern Molecular biology Extracellular Matrix medicine.anatomical_structure Gene Expression Regulation chemistry biology.protein Collagen DNA Probes Protein Processing Post-Translational Procollagen |
| Zdroj: | European Journal of Clinical Investigation. 25:959-966 |
| ISSN: | 1365-2362 0014-2972 |
| DOI: | 10.1111/j.1365-2362.1995.tb01974.x |
| Popis: | Transforming growth factor beta 1 (TGF-beta 1) is an osteotropic growth factor that is found in substantial concentration in bone. The authors studied the influence of TGF-beta 1 on the modification of lysine residues of collagen I. The degree of lysyl hydroxylation and lysyl glycosylation of newly synthesized collagen as well as steady-state levels of mRNA for both lysyl hydroxylase and collagens I and III were determined in human osteoblast-like cells in vitro. In normal human osteoblasts lysyl hydroxylation was decreased by TGF-beta 1 particularly in the collagen alpha 2-chain. This effect was paralleled by an increase in lysyl residues, whereas glycosylation was not affected. The mRNA for lysyl hydroxylase was reduced by one-third under the influence of TGF-beta 1. Additionally, the mRNAs for both procollagen I alpha-chains were stimulated by TGF-beta 1, whereas pro alpha 1 (III)-mRNA showed a decrease. Changes in the local regulatory activity of TGF-beta 1 may play a role in matrix maturation such as collagen type production and lysyl hydroxylation, the latter being altered in various pathological conditions, e.g. in generalized osteopenia. |
| Databáze: | OpenAIRE |
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