Histological and ultrastructural analysis of cryopreserved sheep preantral follicles
| Autor: | Sonia Helena Furtado Costa, Regiane R. Santos, Maria Helena Tavares de Matos, Sônia Nair Báo, José Roberto Viana Silva, Ana Paula Ribeiro Rodrigues, José Ricardo de Figueiredo, Carolina Madeira Lucci, Rob van den Hurk |
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| Rok vydání: | 2006 |
| Předmět: |
Glycerol
Cytoplasm Ethylene Glycol endocrine system Pathology medicine.medical_specialty Hot Temperature Cryoprotectant Biology Cryopreservation Propanediol Andrology chemistry.chemical_compound Cryoprotective Agents Endocrinology Ovarian Follicle Food Animals medicine Animals Dimethyl Sulfoxide Cell Nucleus Sheep Dimethyl sulfoxide Ovarian tissue Ovary General Medicine Microscopy Electron chemistry Propylene Glycols Ultrastructure Female Animal Science and Zoology Tissue Preservation |
| Zdroj: | Animal Reproduction Science. 91:249-263 |
| ISSN: | 0378-4320 |
| Popis: | The aim of this study was to verify the histological and ultrastructural characteristics of sheep preantral follicles after exposure of ovarian tissue to cryopreservation in glycerol (GLY), ethylene glycol (EG), propanediol (PROH) or dimethyl sulfoxide (DMSO) in order to determine the optimum method to store sheep ovarian tissue for later experimental or clinical use. Each ovarian pair from five mixed-breed ewes was divided into 17 fragments. One (control) fragment was immediately fixed for routine histological and ultrastructural studies and the remaining (test) fragments were randomly distributed in cryotubes, equilibrated at 20 degrees C/20 min in 1.8 mL of minimal essential medium (MEM) containing 1.5 or 3 M GLY, EG, PROH or DMSO and then either fixed for morphological studies to determine their possible toxic effect or frozen/thawed and then fixed to test the effect of cryopreservation on preantral follicles. Histological analysis showed that, compared to control fragments, all cryoprotectants at both concentrations significantly reduced the percentage of normal preantral follicles in ovarian fragments prior to or after cryopreservation. PROH 3.0 M appeared to exert a more toxic effect (P |
| Databáze: | OpenAIRE |
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