A TILLING platform for functional genomics in [i]Brachypodium distachyon[/i]
| Autor: | Nicolas Oria, Richard Sibout, Herman Höfte, Véronique Brunaud, Abdelafid Bendahmane, Christelle Troadec, Eddy Blondet, Catherine Lapierre, Laurent Cézard, Olivier Darracq, Lise Jouanin, Frédéric Legée, Madeleine Bouvier d’Yvoire, Marion Dalmais, Sébastien Antelme, Séverine Ho-Yue-Kuang, Yin Wang |
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| Přispěvatelé: | Unité de recherche en génomique végétale (URGV), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, The European FP7 project n° 211982 'RENEWALL' and the Agence Nationale de la Recherche (ANR) projects ANR-08-KBBE-004 'CELLWALL' and ANR-10-BLAN-1528 'PHENOWALL', Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
TILLING Models Molecular Mutation rate [SDV]Life Sciences [q-bio] Mutant lcsh:Medicine Plant Science 01 natural sciences Lignin brachypodium distachyon Plant Genomics lcsh:Science Phylogeny Plant Proteins Genetics 0303 health sciences education.field_of_study Multidisciplinary biology food and beverages Genomics Phenotype Brachypodium Brachypodium distachyon Functional genomics functional genomics Genome Plant Research Article Evolutionary Processes Population 03 medical and health sciences Model Organisms education Biology 030304 developmental biology Evolutionary Biology lcsh:R Computational Biology Sequence Analysis DNA biology.organism_classification Biosynthetic Pathways Plant Breeding Mutation lcsh:Q Plant Biotechnology Population Genetics 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (6), ⟨10.1371/journal.pone.0065503⟩ PLoS ONE, 2013, 8 (6), ⟨10.1371/journal.pone.0065503⟩ PLoS ONE, Vol 8, Iss 6, p e65503 (2013) Plos One 6 (8), . (2013) |
| ISSN: | 1932-6203 |
| Popis: | The new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool for functional genomics. We have established a sodium azide-induced mutant collection and a TILLING platform, called "BRACHYTIL", for the inbred line Bd21-3. The TILLING collection consists of DNA isolated from 5530 different families. Phenotypes were reported and organized in a phenotypic tree that is freely available online. The tilling platform was validated by the isolation of mutants for seven genes belonging to multigene families of the lignin biosynthesis pathway. In particular, a large allelic series for BdCOMT6, a caffeic acid O-methyl transferase was identified. Some mutants show lower lignin content when compared to wild-type plants as well as a typical decrease of syringyl units, a hallmark of COMT-deficient plants. The mutation rate was estimated at one mutation per 396 kb, or an average of 680 mutations per line. The collection was also used to assess the Genetically Effective Cell Number that was shown to be at least equal to 4 cells in Brachypodium distachyon. The mutant population and the TILLING platform should greatly facilitate functional genomics approaches in this model organism. |
| Databáze: | OpenAIRE |
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