Kinetics of infective juvenile production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture
| Autor: | Martha Gayosso-Canales, Marco-Antonio Islas-López, Gabriela Maciel-Vergara, Norberto Chavarría-Hernández, Adriana-Inés Rodríguez-Hernández |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
biology
Nematoda Kinetics Cell Culture Techniques Bioengineering General Medicine Entomopathogenic nematode Agave biology.organism_classification Models Biological Xenorhabdus Horticulture Bioreactors Botany Juvenile Yeast extract Animals Computer Simulation Pest Control Biological Steinernema carpocapsae Corn oil Biotechnology Production rate |
| Zdroj: | Bioprocess and biosystems engineering. 31(5) |
| ISSN: | 1615-7591 |
| Popis: | The effects of culture medium formulations on the kinetics of infective juvenile (IJ) production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture, were studied at the cylindrical-bottle scale using six culture media containing agave juice from Agave spp. among other ingredients. The IJ production kinetics was well modelled through a re-parameterised 3-parameter Gompertz model with kinetic parameters: IJ-lag phase λ IJ (day), maximum IJ-stage production rate m max (day−1), and IJ-multiplication factor (C IJ/C IJ,0)max(−). The variation of λ IJ was not very important within fermentations (10.3–16.2 days); nonetheless, important effects were observed on m max (32.8–241.2 days−1) and (C IJ/C IJ,0)max (66(−) to 611.4(−)). Particularly, maximum values of m max and (C IJ/C IJ,0)max were obtained in medium A4 (0.276 l l−1 agave juice, 17 g l−1 yeast extract, 12 g l−1 dried egg yolk, 0.025 l l−1 corn oil). Also, the maximum IJ concentration (249,444 per ml) was achieved in A4-fermentations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink