Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane ofArabidopsis thaliana
| Autor: | Yan Wang, Lu Li, Jordan D. Radomiljac, Szymon Kubiszewski-Jakubiak, Oliver Berkowitz, Monika W. Murcha, Simon R. Law, Owen Duncan, Reena Narsai, Olivier Keech, James Whelan |
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| Rok vydání: | 2016 |
| Předmět: |
Chlorophyll
0106 biological sciences 0301 basic medicine Aging Chloroplasts senescence Voltage-dependent anion channel Physiology membrane transport Translocase of the outer membrane Arabidopsis Saccharomyces cerevisiae Plant Science Mitochondrial Membrane Transport Proteins Polymerase Chain Reaction β-barrel protein 01 natural sciences Gene Knockout Techniques 03 medical and health sciences Mitochondrial membrane transport protein At3g27930 OM47 Sorting and assembly machinery VDAC biology Arabidopsis Proteins Botany food and beverages Botanik Membrane transport Mitochondria Cell biology Plant Leaves mitochondria Chloroplast 030104 developmental biology Biochemistry Mutation Translocase of the inner membrane biology.protein Bacterial outer membrane Research Paper 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erw366 |
| Popis: | Highlight Characterization of a novel β-barrel protein in Arabidopsis identifies its role in leaf senescence by mediating the transport of chloroplast breakdown products across the outer mitochondrial membrane for their recycling. In plant cells, mitochondria are major providers of energy and building blocks for growth and development as well as abiotic and biotic stress responses. They are encircled by two lipid membranes containing proteins that control mitochondrial function through the import of macromolecules and metabolites. Characterization of a novel β-barrel protein, OUTER MEMBRANE PROTEIN 47 (OM47), unique to the green lineage and related to the voltage-dependent anion channel (VDAC) protein family, showed that OM47 can complement a VDAC mutant in yeast. Mutation of OM47 in Arabidopsis thaliana by T-DNA insertion had no effect on the import of proteins, such as the β-barrel proteins translocase of the outer membrane 40 (TOM40) or sorting and assembly machinery 50 (SAM50), into mitochondria. Molecular and physiological analyses revealed a delay in chlorophyll breakdown, higher levels of starch, and a delay in the induction of senescence marker genes in the mutant lines. While there was a reduction of >90% in OM47 protein in mitochondria isolated from 3-week-old om47 mutants, in mitochondria isolated from 8-week-old plants OM47 levels were similar to that of the wild type. This recovery was achieved by an up-regulation of OM47 transcript abundance in the mutants. Combined, these results highlight a role in leaf senescence for this plant-specific β-barrel protein, probably mediating the recovery and recycling of chloroplast breakdown products by transporting metabolic intermediates into and out of mitochondria. |
| Databáze: | OpenAIRE |
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