Detection of 'Hidden' Antimicrobial Drug Resistance
| Autor: | Qingfeng Hu, Xingrou Chen, Dongshi Gu, Christopher G. Dowson, Yan Yu, Ning Xu, Jennifer Ruan, Li Xie, Benfang Helen Ruan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Bacteriological Techniques Diagnostic methods Bacteria business.industry 030106 microbiology Tetrazolium Salts Microbial Sensitivity Tests Antimicrobial drug Anti-Bacterial Agents High-Throughput Screening Assays 03 medical and health sciences 030104 developmental biology Infectious Diseases Clinical diagnosis Drug Resistance Multiple Bacterial Escherichia coli Medicine Humans business Intensive care medicine |
| Zdroj: | ACS infectious diseases. 5(7) |
| ISSN: | 2373-8227 |
| Popis: | Antimicrobial drug resistance has become a serious public health problem. The current clinical diagnostic methods are turbidity-based assays that have been used for years to track bacterial growth; however, the method is relatively insensitive. To eliminate the new occurrence of drug resistance in infectious bacteria, we developed a highly sensitive EZMTT method for the antibiotic susceptibility test (AST) that magnified the cell growth signal and revealed partial drug resistance (showing 2-20% weak cell growth) that was not detected by the current turbidity assay within 24 h. By simply mixing the EZMTT dye with the bacterial culture and then following the growth by absorbance measurement at 450 nm, the drug-induced proliferation (DIP) rate can be obtained in a high-throughput-screening (HTS) mode with greater than 10-fold better sensitivity than the turbidity assay. The EZMTT-based DIP rate assay of 5 clinically isolated |
| Databáze: | OpenAIRE |
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