Role of angiotensin II in the brain inflammatory events during experimental diabetes in rats
| Autor: | Ninoska Viera, Adriana Pedreañez, Renata Vargas, Jesús Mosquera, Caterina Peña, Juan Pablo Hernández-Fonseca, Jaimar Rincon |
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| Rok vydání: | 2012 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty CD8 Antigens Monocytes Receptor Angiotensin Type 1 Diabetes Mellitus Experimental Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Diabetic Neuropathies Superoxides Diabetes mellitus Internal medicine Renin–angiotensin system medicine Animals Enalapril Molecular Biology Inflammation Angiotensin II receptor type 1 business.industry Angiotensin II Macrophages General Neuroscience Brain Catalase Intercellular Adhesion Molecule-1 medicine.disease Streptozotocin Lymphocyte Function-Associated Antigen-1 Rats Endocrinology Losartan chemistry Neurology (clinical) business Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 1453:64-76 |
| ISSN: | 0006-8993 |
| Popis: | Hyperglycemia during diabetes is one of the causes of encephalopathy. However, diabetes causes chronic inflammatory complications and among them is peripheral neuropathy. Since, diabetes is one of the major risk factors for cerebrovascular disease, inflammatory process could take place in central nervous system (CNS). To test that hypothesis, experiments to determine inflammatory events in CNS during streptozotocin-induced diabetes were performed. Diabetes was induced by intravenous injection of streptozotocin (STZ). Brain angiotensin II (Ang II), monocyte/macrophage (ED-1 positive cells), CD8, the intercellular adhesion molecule-1 (ICAM-1), the lymphocyte function-associated antigen-1 (LFA-1) and superoxide anion were determined by hystochemical and immunohistochemical methods. Nitric oxide (NO), malondialdehyde (MDA) and catalase activity were measured in brain homogenates by enzymatic and biochemical methods. This research showed increased expressions of Ang II, ICAM-1, LFA-1 and CD8 positive cells in diverse zones of cerebrum and cerebellum of diabetic rats (week 8). Treatment of diabetic animals with losartan or enalapril reduced the expression of those molecules. Values of lipid peroxidation, nitrite content and superoxide anion expression remained similar to control rats. Only decreased activity of catalase was observed in diabetic animals, but losartan or enalapril failed to modify catalase activity. This study suggests the presence of Ang II-mediated brain inflammatory events in diabetes probably mediated by AT1 receptors. |
| Databáze: | OpenAIRE |
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