Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells
| Autor: | Jianchun Cai, Yi-zhuo Lu, Guo-yang Wu, Liang-hui Li, Huiqin Zhuo, Guoyan Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Gastric cancer
03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine PI3K/Akt signaling pathway Medicine LY294002 030212 general & internal medicine Phosphorylation PI3K/AKT/mTOR pathway Pi3k akt signaling 030505 public health Akt/PKB signaling pathway business.industry lcsh:Public aspects of medicine Public Health Environmental and Occupational Health lcsh:RA1-1270 chemistry MK-2206 Apoptosis Cancer cell Cancer research Original Article 0305 other medical science business PRAS40-Thr246 |
| Zdroj: | Iranian Journal of Public Health Iranian Journal of Public Health, Vol 48, Iss 12 (2019) |
| ISSN: | 2251-6093 2251-6085 |
| Popis: | Background: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells. Methods: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly. Results: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway. Conclusion: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors. |
| Databáze: | OpenAIRE |
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