Absence of curli in soil-persistent Escherichia coli is mediated by a C-di-GMP signaling defect and suggests evidence of biofilm-independent niche specialization
| Autor: | Leighton Pritchard, Florence Abram, Fiona Brennan, Yinka Somorin, Conor P. O'Byrne, Tara L. Vollmerhausen, Nicholas R. Waters |
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| Přispěvatelé: | Thomas Crawford Hayes Research Award, NUI Galway College of Science |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) RM salmonella 030106 microbiology Fimbria Mutant lcsh:QR1-502 surfaces Biology medicine.disease_cause curli fimbriae complex mixtures Microbiology lcsh:Microbiology biofilm soil o157h7 03 medical and health sciences RpoS expression medicine Escherichia coli pseudomonas-fluorescens Pathogen attachment Wild type Biofilm temperature c-di-GMP cellulose QR 030104 developmental biology gastrointestinal-tract rpoS Soil microbiology |
| Zdroj: | Frontiers in microbiology Somorin, Y, Vollmerhausen, T, Waters, N, Pritchard, L, Abram, F, Brennan, F & O’Byrne, C 2018, ' Absence of curli in soil-persistent Escherichia coli is mediated by a C-di-GMP signaling defect and suggests evidence of biofilm-independent niche specialization ', Frontiers in Microbiology, vol. 9, 1340, pp. 1-13 . https://doi.org/10.3389/fmicb.2018.01340 Frontiers in Microbiology, Vol 9 (2018) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2018.01340 |
| Popis: | peer-reviewed Escherichia coli is commonly viewed as a gastrointestinal commensal or pathogen although an increasing body of evidence suggests that it can persist in non-host environments as well. Curli are a major component of biofilm in many enteric bacteria including E. coli and are important for adherence to different biotic and abiotic surfaces. In this study we investigated curli production in a unique collection of soil-persistent E. coli isolates and examined the role of curli formation in environmental persistence. Although most soil-persistent E. coli were curli-positive, 10% of isolates were curli-negative (17 out of 170). Curli-producing E. coli (COB583, COB585, and BW25113) displayed significantly more attachment to quartz sand than the curli-negative strains. Long-term soil survival experiments indicated that curli production was not required for long-term survival in live soil (over 110 days), as a curli-negative mutant BW25113ΔcsgB had similar survival compared to wild type BW25113. Mutations in two genes associated with c-di-GMP metabolism, dgcE and pdeR, correlated with loss of curli in eight soil-persistent strains, although this did not significantly impair their survival in soil compared to curli-positive strains. Overall, the data indicate that curli-deficient and biofilm-defective strains, that also have a defect in attachment to quartz sand, are able to reside in soil for long periods of time thus pointing to the possibility that niches may exist in the soil that can support long-term survival independently of biofilm formation. |
| Databáze: | OpenAIRE |
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