Chagas' disease in Aboriginal and Creole communities from the Gran Chaco Region of Argentina: Seroprevalence and molecular parasitological characterization

Autor: Carolina Cura, Laura Belén Formichelli, Alejandro G. Schijman, D. O. Hernández, Bettina Laura Brusés, Gerardo Daniel Deluca, Raul Horacio Lucero, Susana Alicia Besuschio, Margarita Bisio, G. J. Fernández, Natalia Anahí Juiz
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Rural Population
Veterinary medicine
Endemic Diseases
Ciencias de la Salud
Parasite load
Parasite Load
Serology
0302 clinical medicine
Seroepidemiologic Studies
Parasite hosting
Child
Phylogeny
Ecology
DNA
Kinetoplast

Middle Aged
Infectious Diseases
Child
Preschool

Ctab Reagent
Polymerase Chain Reaction

purl.org/becyt/ford/3 [https]
Microbiology (medical)
Chagas disease
Adult
CIENCIAS MÉDICAS Y DE LA SALUD
Adolescent
Aboriginal Population
Trypanosoma cruzi
030231 tropical medicine
Creole language
Argentina
Biology
Microbiology
Vulnerable Populations
03 medical and health sciences
Parasitic Load
Trypanosoma Cruzi
Discrete Typing Unit

purl.org/becyt/ford/3.3 [https]
parasitic diseases
Genetics
medicine
Seroprevalence
Humans
Chagas Disease
Molecular Biology
Ecology
Evolution
Behavior and Systematics

Aged
Indians
South American

Infant
medicine.disease
biology.organism_classification
Enfermedades Infecciosas
030104 developmental biology
Rural area
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Most indigenous ethnias from Northern Argentina live in rural areas of "the Gran Chaco" region, where Trypanosoma cruzi is endemic. Serological and parasitological features have been poorly characterized in Aboriginal populations and scarce information exist regarding relevant T. cruzi discrete typing units (DTU) and parasitic loads. This study was focused to characterize T. cruzi infection in Qom, Mocoit, Pit'laxá and Wichi ethnias (N = 604) and Creole communities (N = 257) inhabiting rural villages from two highly endemic provinces of the Argentinean Gran Chaco.DNA extracted using Hexadecyltrimethyl Ammonium Bromide reagent from peripheral blood samples was used for conventional PCR targeted to parasite kinetoplastid DNA (kDNA) and identification of DTUs using nuclear genomic markers. In kDNA-PCR positive samples from three rural Aboriginal communities of "Monte Impenetrable Chaqueño", minicircle signatures were characterized by Low stringency single primer-PCR and parasitic loads calculated using Real-Time PCR.Seroprevalence was higher in Aboriginal (47.98%) than in Creole (27.23%) rural communities (Chi square, p = 4.e-8). A low seroprevalence (4.3%) was detected in a Qom settlement at the suburbs of Resistencia city (Fisher Exact test, p = 2.e-21).The kDNA-PCR positivity was 42.15% in Aboriginal communities and 65.71% in Creole populations (Chi square, p = 5.e-4). Among Aboriginal communities kDNA-PCR positivity was heterogeneous (Chi square, p = 1.e-4). Highest kDNA-PCR positivity (79%) was detected in the Qom community of Colonia Aborigen and the lowest PCR positivity in two different surveys at the Wichi community of Misión Nueva Pompeya (33.3% in 2010 and 20.8% in 2014).TcV (or TcII/V/VI) was predominant in both Aboriginal and Creole communities, in agreement with DTU distribution reported for the region. Besides, two subjects were infected with TcVI, one with TcI and four presented mixed infections of TcV plus TcII/VI. Most minicircle signatures clustered according to their original localities, but in a few cases, signatures from one locality clustered with signatures from other village, suggesting circulation of the same strains in the area. Parasitic loads ranged from undetectable to around 50 parasite equivalents/mL, showing higher values than those generally observed in chronic Chagas disease patients living in urban centers of Argentina. Our findings reveal the persistence of high levels of infection in these neglected populations. Fil: Lucero, R. H.. Universidad Nacional del Nordeste; Argentina Fil: Brusés, B. L.. Universidad Nacional del Nordeste; Argentina Fil: Cura, Carolina Inés. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Formichelli, L. B.. Universidad Nacional del Nordeste; Argentina Fil: Juiz, Natalia Anahí. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Fernández, G. J.. Universidad Nacional del Nordeste; Argentina Fil: Bisio, Margarita María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina Fil: Deluca, Gerardo Daniel. Universidad Nacional del Nordeste; Argentina Fil: Besuschio, Susana Alicia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Hernández, D. O.. Universidad Nacional del Nordeste; Argentina Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
Databáze: OpenAIRE