The Expression of BNP, ET-1, and TGF-β1 in Myocardium of Rats with Ventricular Arrhythmias
Autor: | Yuan Zhang, Bao-Li Zhu, Y Xiao, Yuqing Jia, Zhipeng Cao, J J Xue, Xin-Yi Luo, Tianqi Wang, Meihui Tian |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Endothelin Receptor Antagonists
Male Gene Expression 030204 cardiovascular system & hematology scd Sudden cardiac death Rats Sprague-Dawley lcsh:Chemistry 0302 clinical medicine Natriuretic Peptide Brain Receptor lcsh:QH301-705.5 Spectroscopy bnp Endothelin-1 medicine.diagnostic_test Receptors Endothelin General Medicine Brain natriuretic peptide Computer Science Applications medicine.anatomical_structure Real-time polymerase chain reaction 030220 oncology & carcinogenesis Benzamides cardiovascular system Immunohistochemistry Oligopeptides medicine.medical_specialty Dioxoles Article Catalysis Transforming Growth Factor beta1 Inorganic Chemistry 03 medical and health sciences Western blot Internal medicine medicine Animals cardiovascular diseases Physical and Theoretical Chemistry Molecular Biology et-1 ventricular arrhythmia business.industry Myocardium Organic Chemistry tgf-β1 Arrhythmias Cardiac medicine.disease Death Sudden Cardiac Endocrinology lcsh:Biology (General) lcsh:QD1-999 Ventricle business Receptors Transforming Growth Factor beta Transforming growth factor |
Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 23, p 5845 (2019) International Journal of Molecular Sciences Volume 20 Issue 23 |
ISSN: | 1422-0067 |
Popis: | Ventricular arrhythmia (VA) is a major component of sudden cardiac death (SCD). To investigate the expression of brain natriuretic peptide (BNP), endothelin-1 (ET-1), and transforming growth factor-beta 1 (TGF-&beta 1) during VA, we established a rat model of VA induced by BaCl2 solution through a microinjector pump. PD142893 (ET-1 receptor blocker) and SB431542 (TGF-&beta 1 receptor type I blocker) were used to explore the effect of ET-1 and TGF-&beta 1 on BNP expression in the myocardium after VA. BNP, ET-1, and TGF-&beta 1 in rat myocardium were assayed by western blot and immunohistochemical staining for proteins, and real-time quantitative polymerase chain reaction for mRNAs. We found increased expression of BNP and ET-1 in rat myocardium that was associated with the duration of VA. However, TGF-&beta 1 protein expression remained unchanged. Such early increases in BNP and ET-1 may be attributed to fatal arrhythmias associated with SCD, suggesting these may be novel biomarkers of this disease. After intraperitoneal injection of PD142893 and SB431542, respectively, BNP was downregulated in the myocardium of the left ventricle however, this was abrogated by co-application of the two inhibitors. These results suggested that both ET-1 and TGF-&beta 1, by specifically binding to their receptors, might be involved in the myocardial synthesis of BNP during VA in vivo. |
Databáze: | OpenAIRE |
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