The Expression of BNP, ET-1, and TGF-β1 in Myocardium of Rats with Ventricular Arrhythmias

Autor: Yuan Zhang, Bao-Li Zhu, Y Xiao, Yuqing Jia, Zhipeng Cao, J J Xue, Xin-Yi Luo, Tianqi Wang, Meihui Tian
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Endothelin Receptor Antagonists
Male
Gene Expression
030204 cardiovascular system & hematology
scd
Sudden cardiac death
Rats
Sprague-Dawley
lcsh:Chemistry
0302 clinical medicine
Natriuretic Peptide
Brain
Receptor
lcsh:QH301-705.5
Spectroscopy
bnp
Endothelin-1
medicine.diagnostic_test
Receptors
Endothelin
General Medicine
Brain natriuretic peptide
Computer Science Applications
medicine.anatomical_structure
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Benzamides
cardiovascular system
Immunohistochemistry
Oligopeptides
medicine.medical_specialty
Dioxoles
Article
Catalysis
Transforming Growth Factor beta1
Inorganic Chemistry
03 medical and health sciences
Western blot
Internal medicine
medicine
Animals
cardiovascular diseases
Physical and Theoretical Chemistry
Molecular Biology
et-1
ventricular arrhythmia
business.industry
Myocardium
Organic Chemistry
tgf-β1
Arrhythmias
Cardiac
medicine.disease
Death
Sudden
Cardiac
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Ventricle
business
Receptors
Transforming Growth Factor beta
Transforming growth factor
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 23, p 5845 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 23
ISSN: 1422-0067
Popis: Ventricular arrhythmia (VA) is a major component of sudden cardiac death (SCD). To investigate the expression of brain natriuretic peptide (BNP), endothelin-1 (ET-1), and transforming growth factor-beta 1 (TGF-&beta
1) during VA, we established a rat model of VA induced by BaCl2 solution through a microinjector pump. PD142893 (ET-1 receptor blocker) and SB431542 (TGF-&beta
1 receptor type I blocker) were used to explore the effect of ET-1 and TGF-&beta
1 on BNP expression in the myocardium after VA. BNP, ET-1, and TGF-&beta
1 in rat myocardium were assayed by western blot and immunohistochemical staining for proteins, and real-time quantitative polymerase chain reaction for mRNAs. We found increased expression of BNP and ET-1 in rat myocardium that was associated with the duration of VA. However, TGF-&beta
1 protein expression remained unchanged. Such early increases in BNP and ET-1 may be attributed to fatal arrhythmias associated with SCD, suggesting these may be novel biomarkers of this disease. After intraperitoneal injection of PD142893 and SB431542, respectively, BNP was downregulated in the myocardium of the left ventricle
however, this was abrogated by co-application of the two inhibitors. These results suggested that both ET-1 and TGF-&beta
1, by specifically binding to their receptors, might be involved in the myocardial synthesis of BNP during VA in vivo.
Databáze: OpenAIRE
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