Differentiation of Lactobacillus plantarum, L. pentosus and L. paraplantarum species by RAPD-PCR and AFLP
| Autor: | Sandra Torriani, Francesco Clementi, Franco Dellaglio, Bart Hoste, Karel Kersters, Marc Vancanneyt |
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| Rok vydání: | 2002 |
| Předmět: |
RAPD-PCR
DNA Bacterial AFLP AFLP Genomic relationship Lactobacillus plantarum group RAPD-PCR Taxonomy Genomic relationship Lactobacillus pentosus Lactobacillus paraplantarum Applied Microbiology and Biotechnology Microbiology Restriction fragment Lactobacillus Cluster Analysis Lactobacillus plantarum group Ecology Evolution Behavior and Systematics Phylogeny Taxonomy biology food and beverages Sequence Analysis DNA biology.organism_classification Molecular biology RAPD Random Amplified Polymorphic DNA Technique genomic DNA biology.protein Amplified fragment length polymorphism Lactobacillus plantarum |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0723-2020 |
| Popis: | Two high-resolution genotypic techniques (RAPD-PCR and AFLP) were evaluated for their possibility to discriminate the species Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus paraplantarum and to type these taxa at the infra-species level. In total 23 strains of L. plantarum, three strains of L. pentosus, two strains of L. paraplantarum and two related strains for which the species assignment was not clear, were studied. For RAPD-PCR, suitable oligonucleotides and amplification conditions were selected and tested. For AFLP, a double digest of total genomic DNA was used and a subset of restriction fragments was selectively amplified and visualised using different primer combinations. Both methodologies generated, species-specific electrophoretic profiles. Moreover, the presence of distinct subgroups was revealed within the species L. plantarum. |
| Databáze: | OpenAIRE |
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