Localized 3D analysis of cartilage composition and morphology in small animal models of joint degeneration
| Autor: | Robert E. Guldberg, Hazel Y. Stevens, S. Moran, Angela S.P. Lin, Nick J. Willett, R.V. Kamath, T. Thote, M.A. Hart, Yazdan Raji |
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| Rok vydání: | 2013 |
| Předmět: |
Cartilage
Articular Male Pathology medicine.medical_specialty 3d analysis Biomedical Engineering Degeneration (medical) Osteoarthritis 03 medical and health sciences 0302 clinical medicine Rheumatology Small animal Image Processing Computer-Assisted EPIC-μCT medicine Animals MIA Orthopedics and Sports Medicine Rats Wistar Joint (geology) Cartilage degeneration 030304 developmental biology 030203 arthritis & rheumatology 0303 health sciences Tibia business.industry Cartilage Scoring methods X-Ray Microtomography medicine.disease Arthritis Experimental Iodoacetic Acid Rats Tibial Meniscus Injuries MMT Disease Models Animal medicine.anatomical_structure Disease Progression Rat Proteoglycans Joint degeneration business |
| Zdroj: | Osteoarthritis and Cartilage. 21:1132-1141 |
| ISSN: | 1063-4584 |
| Popis: | Summary Objective Current histological scoring methods to evaluate efficacy of potential therapeutics for slowing or preventing joint degeneration are time-consuming and semi-quantitative in nature. Hence, there is a need to develop and standardize quantitative outcome measures to define sensitive metrics for studying potential therapeutics. The objectives of this study were to use equilibrium partitioning of an ionic contrast agent via Equilibrium Partitioning of an Ionic Contrast-Microcomputed tomography (EPIC-μCT) to quantitatively characterize morphological and compositional changes in the tibial articular cartilage in two distinct models of joint degeneration and define localized regions of interest to detect degenerative cartilage changes. Materials and Methods The monosodium iodoacetate (MIA) and medial meniscal transection (MMT) rat models were used in this study. Three weeks post-surgery, tibiae were analyzed using EPIC-μCT and histology. EPIC-μCT allowed measurement of 3D morphological changes in cartilage thickness, volume and composition. Results Extensive cartilage degeneration was observed throughout the joint in the MIA model after 3 weeks. In contrast, the MMT model showed more localized degeneration with regional thickening of the medial tibial plateau and a decrease in attenuation consistent with proteoglycan (PG) depletion. Focal lesions were also observed and 3D volume calculated as an additional outcome metric. Conclusions EPIC-μCT was used to quantitatively assess joint degeneration in two distinct preclinical models. The MMT model showed similar features to human Osteoarthritis (OA), including localized lesion formation and PG loss, while the MIA model displayed extensive cartilage degeneration throughout the joint. EPIC-μCT imaging provides a rapid and quantitative screening tool for preclinical evaluation of OA therapeutics. |
| Databáze: | OpenAIRE |
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