Inkjet Printed Interdigitated Biosensor for Easy and Rapid Detection of Bacteriophage Contamination: a Preliminary Study for Milk Processing Control Applications
| Autor: | Antonio Del Casale, Sandra Torriani, Alessandro De Toni, Arianna Cunego, M. Scaramuzza, A. Paccagnella, Fabio Fracchetti, Giulio Rosati |
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| Rok vydání: | 2019 |
| Předmět: |
Fabrication
Materials science Nanotechnology 02 engineering and technology 01 natural sciences Silver nanoparticle Analytical Chemistry lcsh:Biochemistry bacteriophage Microscopy Bacteria Bacteriophage Biosensors Electrochemical impedance spectroscopy Impedance Inkjet Interdigitated electrodes Milk lcsh:QD415-436 Physical and Theoretical Chemistry bacteria interdigitated electrodes milk Inkwell 010401 analytical chemistry Response time Contamination biosensors 021001 nanoscience & nanotechnology 0104 chemical sciences Dielectric spectroscopy electrochemical impedance spectroscopy inkjet impedance 0210 nano-technology Biosensor |
| Zdroj: | Chemosensors Volume 7 Issue 1 Chemosensors, Vol 7, Iss 1, p 8 (2019) |
| ISSN: | 2227-9040 |
| Popis: | Bacteriophages are responsible for significant material and time losses in the dairy industry. This because these viruses infect the selected lactic starter cultures used for milk fermentation, i.e., the first stage toward cheese production. Standard detection techniques are time- and labor-consuming, causing huge costs related to production plant sanitation and product wasting. A new type of biosensor for early detection of bacteriophage contamination is highly demanded by the milk processing market, and inkjet-printed electrochemical sensors could be the answer. Inkjet printing is a well-known technology that has been revisited in recent years, using silver nanoparticle (AgNP) based inks for low-cost and easy fabrication of sensing and biosensing systems on flexible and eco-compatible substrates. In this research, we studied inkjet printing for the manufacturing of both interdigitated electrodes arrays (IDEAs), and a versatile system to monitor bacterial cultures by electrochemical impedance spectroscopy (EIS). In particular, we studied this biosensing system for the detection of bacteriophages by comparing its performance with standard microbiological methods. We performed electrical and morphological characterizations of the devices produced with a consumer-use inkjet printer with commercial AgNPs ink on flexible substrates, such as office paper, polyethylene (PET), and photo paper. We used light microscopy optical analysis, profilometry, atomic force microscopy (AFM), and scanning electron microscopy (SEM) imaging to define the objects resolution, their real dimensions, and thickness. We also investigated the devices&rsquo conductivity and layout, by EIS measurements with a standard buffer solution, i.e., phosphate buffered saline (PBS). Finally, we tested our system by monitoring Lactococcus lactis cultures and bacteriophage infection. We compared the results to those obtained by two standard microbiological methods in terms of response time, proving that our technique requires less than half the time of other methods and no specialized personnel. |
| Databáze: | OpenAIRE |
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