Morphological analysis and immunohistochemical expression in restorations with self-adhesive resin: A randomized split-mouth design-controlled study

Autor:	Laís-Maciel Costa, Natália-Gomes de Oliveira, Keylla-Marinho-Albuquerque Barros, Marília-de Lima Soares, Marina-Torreão da Silveira, Leorik-Pereira da Silva, Marianne-de Vasconcelos Carvalho
Rok vydání:	2021
Předmět:	Molar Chemistry business.industry Research Composite number Dentistry Operative Dentistry and Endodontics Self adhesive stomatognathic system Morphological analysis Pulp (tooth) Immunohistochemistry Adhesive Split mouth design business General Dentistry UNESCO:CIENCIAS MÉDICAS
Zdroj:	Journal of Clinical and Experimental Dentistry de Oliveira, N. G., da Silveira, M. T., da Silva, L. P., Barros, K. M., Costa, L. M., Soares, M. L., & Carvalho, M. V. (2021). Morphological analysis and immunohistochemical expression in restorations with self-adhesive resin: A randomized split-mouth design-controlled study. Journal of clinical and experimental dentistry, 13(9), e927–e934.
ISSN:	1989-5488
Popis:	Background To evaluate the morphology and immunohistochemical expression of MMP-9, TIMP-3, COL-I, TNF-alfa and COX-2 in the dentinopulp complex of restorations with self adhesive composite (Vertise Flow/Kerr) compared with conventional resin composite with self-etching adhesive (Filtek Z250/3M ESPE and Clearfil SE Bond/Kuraray-Noritake). Material and Methods A randomized, controlled, double-blind, split-mouth, two-arm clinical trial was conducted. Twenty-seven volunteers with third molars indicated for extraction received two deep class I restorations, one with each material. The morphology and immunohistochemical expression were measured at 15 days after the restorative procesures. The data were submitted to the Fisher`s Exct test (α=0.05). Results Through morphological analysis, a slight disorganization of the odontoblastic layer was visualized, along with a slight inflammatory infiltrate in the VERT and CSEB groups (p< 0.05). In immunohistochemical analysis, TNF-alpha, MMP-9, TIMP-3 and COX-2 demonstrated more cases of positive pulp labeling. Conclusions Self-adhesive resin composite and conventional resin composite with a self-etching bonding agent promoted slight alterations in the dentin-pulp complex, indicating a repair ability and reversibility of the inflammatory process against aggressions of the adhesive restorative procedure. Key words:Immunohistochemistry, inflammation, randomized clinical trial, self-adhering composite, dentistry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15bffe444cb2b62c458c2c04c14c4aff https://pubmed.ncbi.nlm.nih.gov/34603622 Zobrazit plný text záznamu