Resistance to Diamide Insecticides in Plutella xylostella (Lepidoptera: Plutellidae): Comparison Between Lab-Selected Strains and Field-Collected Populations
| Autor: | Huan-Huan Wang, Ying-Ying Wang, Cheng-Hua Wang, Chao Qin, Chao-Bin Xue, Shi-Qing Sun |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine China Insecticides Biology Moths 01 natural sciences Lepidoptera genitalia Insecticide Resistance 03 medical and health sciences chemistry.chemical_compound Cyantraniliprole Animals ortho-Aminobenzoates Amino Acid Sequence Sulfones Cross-resistance Diamondback moth Ecology Indoxacarb Plutella Ryanodine Receptor Calcium Release Channel General Medicine Chlorfenapyr biology.organism_classification 010602 entomology 030104 developmental biology chemistry Plutellidae Insect Science Larva Benzamides Insect Proteins Sequence Alignment |
| Zdroj: | Journal of economic entomology. 111(2) |
| ISSN: | 1938-291X |
| Popis: | Diamondback moth, Plutella xylostella (L.; Lepidoptera: Plutellidae), is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to P. xylostella resistance and this presents a serious threat to vegetable production. We selected chlorantraniliprole (Rf) and flubendiamide (Rh) resistance strains of P. xylostella with resistance ratios of 684.54-fold and 677.25-fold, respectively. The Rf and Rh strains underwent 46 and 36 generations of lab-selection for resistance, respectively. Low cross resistance of Rh to cyantraniliprole was found. Cross resistance to chlorfenapyr, tebufenozid, and indoxacarb was not found in Rf and Rh strains. The P. xylostella ryanodine receptor gene (PxRyR) transcripts level in the Rf and Rh strains was up-regulated. Except for Rf34 and Rh36, PxRyR expression in all generations of Rf and Rh selection gradually increased with increasing resistance. Two resistant populations were field-collected from Guangzhou Baiyun (Rb) and Zengcheng (Rz) and propagated for several generations without exposure to any pesticide had higher PxRyR expression than the susceptible strain (S). In the S strain, PxRyR expression was not related to the resistance ratio. Gene sequencing found that the RyR 4946 gene site was glycine (G) in the S, Rf, and Rh strains, and was glutamate (E) with 70% and 80% frequency in the Rb and Rz populations, respectively. The 4946 gene site was substituted by valine (V) with the frequency of 30% and 20% in Rb and Rz populations, respectively. These results increase the understanding of the mechanisms of diamide insecticide resistance in P. xylostella. |
| Databáze: | OpenAIRE |
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