Cell Sources for Human In vitro Bone Models
| Autor: | Sana Ansari, Sandra Hofmann, Keita Ito |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Osteoclasts Peripheral blood Bone Marrow Cells Biology In Vitro Techniques Models Biological Osteocytes Hematopoietic stem cell Osteocytes (J Delgado-Calle and J Klein-Nulend Section Editors) 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Osteoclast Osteogenesis medicine Humans Bone marrow Progenitor cell Cells Cultured In vitro bone model Cell Proliferation Mesenchymal stem cell Osteoblasts Tissue Engineering Stem Cells Osteoblast Cell Differentiation Personalized medicine Cell biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Osteocyte Stem cell |
| Zdroj: | Current Osteoporosis Reports |
| ISSN: | 1544-1873 |
| DOI: | 10.1007/s11914-020-00648-6 |
| Popis: | Purpose of Review One aim in bone tissue engineering is to develop human cell-based, 3D in vitro bone models to study bone physiology and pathology. Due to the heterogeneity of cells among patients, patient’s own cells are needed to be obtained, ideally, from one single cell source. This review attempts to identify the appropriate cell sources for development of such models. Recent Findings Bone marrow and peripheral blood are considered as suitable sources for extraction of osteoblast/osteocyte and osteoclast progenitor cells. Recent studies on these cell sources have shown no significant differences between isolated progenitor cells. However, various parameters such as medium composition affect the cell’s proliferation and differentiation potential which could make the peripheral blood-derived stem cells superior to the ones from bone marrow. Summary Peripheral blood can be considered a suitable source for osteoblast/osteocyte and osteoclast progenitor cells, being less invasive for the patient. However, more investigations are needed focusing on extraction and differentiation of both cell types from the same donor sample of peripheral blood. |
| Databáze: | OpenAIRE |
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