Pulsed-field gel electrophoresis analysis of Bordetella pertussis populations in various European countries with different vaccine policies

Autor: Frits R. Mooi, Carl Heinz Wirsing von König, Hans O. Hallander, Valérie Caro, Carola Vahrenholz, Elisabeth Njamkepo, Qiushui He, Abdolreza Advani, Marion Riffelmann, Shirley C.M. Van Amersfoorth, Nicole Guiso, Jussi Mertsola
Přispěvatelé: Prévention et Thérapie Moléculaires des Maladies Infectieuses Humaines, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratory for vaccine preventable diseases, National Institute for Public Health and the Environment [Bilthoven] (RIVM), Swedish Institute for Infectious Disease Control, The Pertussis Reference Laboratory, University of Turku-National Public Health Institute, Institut für Hygiene und Laboratoriumsmedizin, Klinikum Krefeld, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2005
Předmět:
DNA
Bacterial
MESH: Chromosomes
Bacterial
Bordetella pertussis
Veterinary medicine
[SDE.MCG]Environmental Sciences/Global Changes
Immunology
Population
Microbiology
MESH: Variation (Genetics)
MESH: Bordetella pertussis
03 medical and health sciences
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Genotype
Pulsed-field gel electrophoresis
Cluster Analysis
Humans
Typing
MESH: Phylogeny
education
Phylogeny
030304 developmental biology
Pertussis Vaccine
0303 health sciences
Genetic diversity
education.field_of_study
MESH: Humans
biology
030306 microbiology
Genetic Variation
Chromosomes
Bacterial
biology.organism_classification
MESH: Cluster Analysis
MESH: DNA
Bacterial
[SDE.ES]Environmental Sciences/Environmental and Society
Electrophoresis
Gel
Pulsed-Field
3. Good health
Europe
Vaccination
MESH: Electrophoresis
Gel
Pulsed-Field
Infectious Diseases
MESH: Pertussis Vaccine
DNA profiling
MESH: Europe
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Zdroj: Microbes and Infection
Microbes and Infection, Elsevier, 2005, 7 (7-8), pp.976-82. ⟨10.1016/j.micinf.2005.04.005⟩
Microbes and Infection, 2005, 7 (7-8), pp.976-82. ⟨10.1016/j.micinf.2005.04.005⟩
ISSN: 1286-4579
DOI: 10.1016/j.micinf.2005.04.005
Popis: The increasing incidence of pertussis in a number of countries, despite good vaccination coverage, is a cause for concern. We used pulsed-field gel electrophoresis (PFGE) typing to examine the genetic diversity of 101 clinical isolates of Bordetella pertussis, recovered during 1999-2001, and circulating in five different European countries to evaluate temporal and geographical distribution. This DNA fingerprinting approach seems to be a more discriminative epidemiological tool than sequencing of individual genes. Despite differences in vaccination policies in the five countries, these European isolates were found to be very similar and fell into the same major PFGE profile groups, with a predominance of one profile group. There was no evidence of geographic clustering, except that one new profile subgroup was predominantly found in one country. This study provides a baseline for continued surveillance of the B. pertussis population in Europe.
Databáze: OpenAIRE
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