Activin B Promotes Initiation and Development of Hair Follicles in Mice
| Autor: | Lu Zhang, Weiya Lang, Min Zhang, Qin Jia, Lei Zhang, Yinghua Chen, Xueer Wang, Shixuan Chen, Yanan Kong, Lin Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Histology H&E stain Biology Tissue Culture Techniques Andrology Mice Random Allocation chemistry.chemical_compound Internal medicine otorhinolaryngologic diseases medicine Animals Oil Red O Dose-Response Relationship Drug integumentary system Regeneration (biology) Hair follicle Activins Staining Mice Inbred C57BL medicine.anatomical_structure Endocrinology chemistry Apoptosis Alkaline phosphatase Female sense organs Anatomy Wound healing Hair Follicle |
| Zdroj: | Cells Tissues Organs. 198:318-326 |
| ISSN: | 1422-6421 1422-6405 |
| DOI: | 10.1159/000356304 |
| Popis: | Activin B has been reported to promote the regeneration of hair follicles during wound healing. However, its role in the development and life cycle of hair follicles has not been elucidated. In our study, the effect of activin B on mouse hair follicles of cultured and neonatal mouse skin was investigated. In these models, PBS or activin B (5, 10 or 50 ng/ml) was applied, and hair follicle development was monitored. Hair follicle initiation and development was examined using hematoxylin and eosin staining, alkaline phosphatase activity staining, Oil Red O+ staining, and the detection of TdT-mediated dUTP-biotin nick end-labeling cell apoptosis. Activin B was found to efficiently induce the initiation of hair follicles in the skin of both cultured and neonatal mice and to promote the development of hair follicles in neonatal mouse skin. Moreover, activin-B-treated hair follicles were observed to enter the anagen stage from the telogen stage and to remain in the anagen stage. These results demonstrate that activin B promotes the initiation and development of hair follicles in mice. |
| Databáze: | OpenAIRE |
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