Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm
| Autor: | Joana Montezano Marques, Juliana Pacheco da Rosa, Samyra Raquel Gonçalves Tiburcio, Lucy Seldin, Rosalie Reed Rodrigues Coelho |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Desulfovibrio alaskensis Biocide lcsh:QR1-502 Microbial Sensitivity Tests Streptomyces Microbiology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration Most probable number Antibiosis Environmental Microbiology Antimicrobial substance Sulfate Sulfate-reducing bacteria biology Sulfates Biofilm Biocorrosion biology.organism_classification Sulfate reducing bacteria Anti-Bacterial Agents 030104 developmental biology chemistry Biofilms Oxidation-Reduction |
| Zdroj: | Brazilian Journal of Microbiology, Volume: 47, Issue: 3, Pages: 603-609, Published: SEP 2016 Brazilian Journal of Microbiology, Vol 47, Iss 3, Pp 603-609 Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.47 n.3 2016 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| ISSN: | 1517-8382 |
| DOI: | 10.1016/j.bjm.2016.04.013 |
| Popis: | Streptomyces lunalinharesii strain 235 produces an antimicrobial substance that is active against sulfate reducing bacteria, the major bacterial group responsible for biofilm formation and biocorrosion in petroleum reservoirs. The use of this antimicrobial substance for sulfate reducing bacteria control is therefore a promising alternative to chemical biocides. In this study the antimicrobial substance did not interfere with the biofilm stability, but the sulfate reducing bacteria biofilm formation was six-fold smaller in carbon steel coupons treated with the antimicrobial substance when compared to the untreated control. A reduction in the most probable number counts of planktonic cells of sulfate reducing bacteria was observed after treatments with the sub-minimal inhibitory concentration, minimal inhibitory concentration, and supra-minimal inhibitory concentration of the antimicrobial substance. Additionally, when the treated coupons were analyzed by scanning electron microscopy, the biofilm formation was found to be substantially reduced when the supra-minimal inhibitory concentration of the antimicrobial substance was used. The coupons used for the biofilm formation had a small weight loss after antimicrobial substance treatment, but corrosion damage was not observed by scanning electron microscopy. The absence of the dsrA gene fragment in the scraped cell suspension after treatment with the supra-minimal inhibitory concentration of the antimicrobial substance suggests that Desulfovibrio alaskensis was not able to adhere to the coupons. This is the first report on an antimicrobial substance produced by Streptomyces active against sulfate reducing bacteria biofilm formation. The application of antimicrobial substance as a potential biocide for sulfate reducing bacteria growth control could be of great interest to the petroleum industry. |
| Databáze: | OpenAIRE |
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