Determination of fatty acids and stable carbon isotopic ratio in subcutaneous fat to identify the feeding regime of Iberian pigs
Autor: | M. Calderón-Santiago, Feliciano Priego-Capote, José Manuel Moreno-Rojas, Juan María García-Casco, María Dolores Luque de Castro, Verónica Sánchez de Medina, María Asunción López-Bascón |
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Rok vydání: | 2014 |
Předmět: |
Multivariate statistics
Swine biology.animal_breed Subcutaneous Fat Fatty acid methyl esters Feeding regimes Subcutaneous fat Gas Chromatography-Mass Spectrometry Stable carbon isotopes Animal science Iberian pig Animals GC−FID Animal nutrition ROC analysis Carbon Isotopes biology Fatty Acids General Chemistry IRMS Animal Feed humanities Feeding regime Isotopic ratio Biochemistry General Agricultural and Biological Sciences |
Zdroj: | Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA INIA: Repositorio de Resultados de Investigación del INIA |
ISSN: | 1520-5118 |
Popis: | Discrimination among the types of feeding regimes for Iberian pigs is currently a highly demanded challenge by the Iberian pig sector. In the present research, discrimination among feeding regimes has been achieved by the combination of two analytical methods (based on FAMEs analysis by GC-FID and determination of δ13C by IRMS) previously used independently without success. In the present study, 80 samples of adipose tissue from Iberian pigs subjected to four different feedings were analyzed. The study of the variables more influenced by the feeding regime has allowed us to configure panels of markers with predictive power for the studied feedings by multivariate ROC analysis. The results provided values of specificity and sensitivity higher than 85% in most cases. The statistical combination of results from different analytical methods could be the key to develop models for the correct discrimination of Iberian pigs according to the feeding regime. © 2014 American Chemical Society. |
Databáze: | OpenAIRE |
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