Microspheres Made of Poly(ɛ-caprolactone)-Based Amphiphilic Copolymers: Potential in Sustained Delivery of Proteins
| Autor: | Luisanna Ostacolo, Rosario Palumbo, Giuseppe De Rosa, Giovanni Maglio, Fabiana Quaglia, Giuseppe Nese, Maria Immacolata La Rotonda |
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| Přispěvatelé: | Quaglia, Fabiana, Ostacolo, Luisanna, Nese, Giuseppe, DE ROSA, Giuseppe, LA ROTONDA, MARIA IMMACOLATA, Palumbo, Rosario, Maglio, Giovanni |
| Rok vydání: | 2005 |
| Předmět: |
Microsphere
Magnetic Resonance Spectroscopy Polymers and Plastics Polymers Polyesters Biocompatible Materials Bioengineering Biomaterials Lactones Crystallinity chemistry.chemical_compound Block Copolymer Polymer chemistry Amphiphile Materials Chemistry Copolymer Animals Particle Size Microparticle Caproates Fluorescent Dyes chemistry.chemical_classification Drug Carriers Microscopy Confocal Chemistry Serum Albumin Bovine Polymer Macromonomer Poly(caprolactone Microspheres Cattle Drug carrier Caprolactone Fluorescein-5-isothiocyanate Biotechnology |
| Zdroj: | Macromolecular Bioscience. 5:945-954 |
| ISSN: | 1616-5195 1616-5187 |
| Popis: | Microspheres of amphiphilic multi-block poly(ester-ether)s (PEE)s and poly(ester-ether-amide)s (PEEA)s based on poly(epsilon-caprolactone) (PCL) were investigated as delivery systems for proteins. The interest was mainly focused on the effect of their molecular structure and composition on the overall properties of the microspheres, encapsulating bovine serum albumin (BSA) as a model protein. PEEs and PEEAs were prepared using a alpha,omega-dihydroxy-terminated PCL macromer (Mn= 2.0 kDa) as a hydrophobic component. Hydrophilic oxyethylene sequences were generated using poly(ethylene oxide)s (PEO)s of different molecular mass (Mn= 300-600 Da) in the case of PEEs, or 4,7,10-trioxa-1,13-tridecanediamine (Trioxy) and PEO150 (Mn= 150 Da) in the case of PEEAs. The copolymers showed a decrease of Tm and crystallinity values as compared with PCL. Within each class of copolymers, the bulk hydrophilicity increased with increasing the number of oxyethylene groups in the chain repeat unit. PEEAs were more hydrophilic than PEEs with a similar number of oxyethylene groups. Discrete spherical particles were prepared by both PEEs and PEEAs and their BSA encapsulation efficiency related to copolymer properties. Interestingly, the insertion of short hydrophilic segments is enough to significantly affect protein distribution inside microspheres and its release profiles, as compared to PCL microspheres. Different degradation rates and mechanisms were observed for copolymer microspheres, mainly depending on the distribution of oxyethylene units along the chain. The results highlight that a fine control over the structural parameters of amphiphilic PCL-based multi-block copolymers is a key factor for their application in the field of protein delivery. |
| Databáze: | OpenAIRE |
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