Cholesterol imprinted composite membranes for selective cholesterol recognition from intestinal mimicking solution
| Autor: | Neşe Hayat Aksoy, Mehmet Odabaşı, Demet Erdönmez, Gözde Baydemir, Lokman Uzun, Ömür Acet |
|---|---|
| Přispěvatelé: | Fen-Edebiyat Fakültesi Veteriner Fakültesi, [Odabasi, Mehmet -- Acet, Orntir] Aksaray Univ, Dept Chem, Aksaray, Turkey -- [Uzun, Lokman] Hacettepe Univ, Dept Chem, Ankara, Turkey -- [Baydemir, Gozde] Adana Sci & Technol Univ, Dept Bioengn, Adana, Turkey -- [Aksoy, Nese Hayat] Aksaray Univ, Dept Biochem, Fac Vet Med, Aksaray, Turkey -- [Erdonmez, Demet] Aksaray Univ, Dept Biol, Ankara, Turkey |
| Rok vydání: | 2018 |
| Předmět: |
N-methacryloyl-(L)-tryptophan (MTrp)
02 engineering and technology Molecularly imprinted polymers (MIP) 01 natural sciences Molecular Imprinting Particles Embedding Colloid and Surface Chemistry Adsorption Biomimetics Spectroscopy Fourier Transform Infrared medicine Intestinal Mucosa Physical and Theoretical Chemistry Fourier transform infrared spectroscopy Chemistry 010401 analytical chemistry Molecularly imprinted polymer Membranes Artificial Surfaces and Interfaces General Medicine Cholesterol Imprinting 021001 nanoscience & nanotechnology 0104 chemical sciences Solutions Cholesterol Membrane Polymerization NIP Swelling medicine.symptom 0210 nano-technology Selectivity Biotechnology Nuclear chemistry |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 163:266-274 |
| ISSN: | 0927-7765 |
| Popis: | WOS: 000427217300031 PubMed: 29316524 Molecularly imprinted polymers which have been extensively investigated as selective adsorbents were constructed using a template molecule during the polymerization to gain template-specific cavities. In this study, we prepared cholesterol imprinted poly(2-hydroxyethyl methacrylate-methacryloyamidotryptophan) (PHEMA-MTrp) particles embedded composite membranes. These membranes were characterized through elemental analysis, FTIR, SEM, swelling tests, and surface area measurements. Adsorption experiments were performed in a batch experimental set-up, and the adsorption medium was either a methanol or intestinal-mimicking solution. Stigmasterol and estradiol were used as competing molecules in selectivity tests. Some results are as follows: the specific surface areas of MIP particle-embedded membranes, NIP particle-embedded membranes, and membranes without particles were 36.5, 29.2 and 13.7 m(2)/g, respectively. The imprinted membranes were 1.96 and 2.13 times more selective for cholesterol when compared to stigmasterol and estradiol used as competitor agents, respectively. Cholesterol adsorption capacity increased up to 23.43 mg/g with increasing cholesterol concentration of 2 mg/mL. The MIP particle-embedded composite membranes showed a negligible loss in cholesterol adsorption capacity after ten consecutive adsorption cycles using the same adsorbent. (C) 2017 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect