Protein patterns ofStreptomyces griseus strains during development
| Autor: | S. Vitális, G. Szabó, G. Valu, I. Békési |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Spores
Bacterial Developmental stage Pulse labelling biology Strain (chemistry) fungi Streptomyces griseus General Medicine Low specific activity biology.organism_classification Applied Microbiology and Biotechnology Spore Bacterial Proteins Biochemistry Mutation Streptomycin Genetics Protein biosynthesis Electrophoresis Polyacrylamide Gel Mycelium |
| Zdroj: | Zeitschrift für allgemeine Mikrobiologie. 24:201-209 |
| ISSN: | 1521-4028 0044-2208 |
| DOI: | 10.1002/jobm.3630240315 |
| Popis: | Protein patterns from mycelial extracts of Streptomyces griseus strains No. 45H and No. 52-1 were studied by one dimensional gradient PAGE with 20 cm run. The results are reproducible, the protein patterns are the same for the same developmental stage in a given strain. There are well-defined characteristic changes of the protein patterns during the life cycle. The proteins of spores show conspicuous differences compared to protein patterns of the old (72 h) mycelia in the same culture. There is no difference between protein patterns of spores from submerged and from solid media. The protein patterns of two closely related Streptomyces griseus strains significantly differ from each other. After pulse labelling with (14C)-labelled protein hydrolyzate for 40 min, the specific activities of the proteins show considerable differences. There are characteristic bands with low specific activity and others with high incorporation. The fluorograms of the 16, 40 and 64 h cultures show that different proteins are being synthesized intensively at different ages of the culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text