Characterization of Aureobasidium pullulans isolated from airborne spores in Thailand
| Autor: | S. Pichayangkura, Sehanat Prasongsuk, Hunsa Punnapayak, M. Sudhadham |
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| Rok vydání: | 2003 |
| Předmět: |
Sucrose
Molecular Sequence Data Air Microbiology Bioengineering Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Ascomycota Polysaccharides DNA Ribosomal Spacer Amylase Food science Internal transcribed spacer DNA Fungal Mycological Typing Techniques Glucans biology Pullulanase Fungal genetics Pullulan Sequence Analysis DNA Spores Fungal Thailand biology.organism_classification Spore Aureobasidium pullulans chemistry biology.protein Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 30:89-94 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-002-0016-y |
| Popis: | Isolates from air in several locations in Thailand were identified as Aureobasidium pullulans PR with dark pigmentation (Loei province), A. pullulans SU with an unusual conidial apparatus (Chiangmai province), and A. pullulans CU with burgundy-red pigmentation (from a shady area in Bangkok). The internal transcribed spacer sequences of the rDNA of A. pullulans SU and A. pullulans CU confirmed that they were A. pullulans. Both A. pullulans CU and A. pullulans PR preferred 30 degrees C and pH 7.5 for exopolysaccharide (EPS) production, while A. pullulans SU preferred 25 degrees C and pH 6.5. All three isolates preferred glucose over sucrose and (NH(4))(2)SO4 over peptone for EPS production. Under optimal conditions, A. pullulans PR produced EPS yields of up to 0.225 g g(-1), followed by A. pullulans CU (0.185 g g(-1)) and A. pullulans SU (0.158 g g(-1)). Amylase activities were detected during the course of EPS production but gradually decreased as the EPS yields increased. IR spectra suggest that the EPS from these isolates was pullulan. EPS from the three isolates were partially sensitive to pullulanase. |
| Databáze: | OpenAIRE |
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