REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum Biomarker
Autor: | Liisa Hattara, Laura Lehtinen, Kristiina Iljin, Pia Roering, Seija Grénman, John Patrick Mpindi, Taina Korpela, Annika Auranen, Katja Kaipio, Johanna Hynninen, Pia Vesterkvist, Caj Haglund, Harri Siitari, Olli Carpén, Ben Davidson |
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Přispěvatelé: | Institute for Molecular Medicine Finland, Olli-Pekka Kallioniemi / Principal Investigator, Clinicum, Translational Cancer Biology (TCB) Research Programme, Department of Surgery, II kirurgian klinikka, Research Programs Unit, Precision Cancer Pathology |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Candidate gene Pathology Databases Factual endocrine system diseases INVASION Cancer Treatment lcsh:Medicine Gene Expression Pancreatitis-Associated Proteins Biochemistry INTESTINAL-TYPE Ovarian tumor 0302 clinical medicine 3123 Gynaecology and paediatrics Medicine and Health Sciences lcsh:Science Ovarian Neoplasms Multidisciplinary Ascites Adenocarcinoma Mucinous TUMORS female genital diseases and pregnancy complications 3. Good health Ovarian Cancer REG4 ovarian cancer Oncology 030220 oncology & carcinogenesis Adenocarcinoma Biomarker (medicine) Immunohistochemistry biomarker Female Endometrial Carcinoma Research Article medicine.medical_specialty TISSUES CARCINOMA 3122 Cancers Gastroenterology and Hepatology Biology ta3111 Carcinomas 03 medical and health sciences WAP Four-Disulfide Core Domain Protein 2 SDG 3 - Good Health and Well-being medicine Carcinoma Biomarkers Tumor Cancer Detection and Diagnosis Genetics Humans Lectins C-Type lcsh:R ta1182 Proteins Cancers and Neoplasms Biology and Life Sciences medicine.disease ta3122 ta3123 PATTERN 030104 developmental biology IV CA-125 Antigen lcsh:Q 3111 Biomedicine Ovarian cancer Gynecological Tumors Biomarkers |
Zdroj: | Lehtinen, L, Vesterkvist, P, Roering, P, Korpela, T, Hattara, L, Kaipio, K, Mpindi, J-P, Hynninen, J, Auranen, A, Davidson, B, Haglund, C, Iljin, K, Grenman, S, Siitari, H & Carpen, O 2016, ' REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum Biomarker ', PLoS ONE, vol. 11, no. 3, e0151590 . https://doi.org/10.1371/journal.pone.0151590 PLoS ONE PLoS ONE, Vol 11, Iss 3, p e0151590 (2016) |
Popis: | Preoperative diagnostics of ovarian neoplasms rely on ultrasound imaging and the serum biomarkers CA125 and HE4. However, these markers may be elevated in non-neoplastic conditions and may fail to identify most non-serous epithelial cancer subtypes. The objective of this study was to identify histotype-specific serum biomarkers for mucinous ovarian cancer. The candidate genes with mucinous histotype specific expression profile were identified from publicly available gene-expression databases and further in silico data mining was performed utilizing the MediSapiens database. Candidate biomarker validation was done using qRT-PCR, western blotting and immunohistochemical staining of tumor tissue microarrays. The expression level of the candidate gene in serum was compared to the serum CA125 and HE4 levels in a patient cohort of prospectively collected advanced ovarian cancer. Database searches identified REG4 as a potential biomarker with specificity for the mucinous ovarian cancer subtype. The specific expression within epithelial ovarian tumors was further confirmed by mRNA analysis. Immunohistochemical staining of ovarian tumor tissue arrays showed distinctive cytoplasmic expression pattern only in mucinous carcinomas and suggested differential expression between benign and malignant mucinous neoplasms. Finally, an ELISA based serum biomarker assay demonstrated increased expression only in patients with mucinous ovarian cancer. This study identifies REG4 as a potential serum biomarker for histotype-specific detection of mucinous ovarian cancer and suggests serum REG4 measurement as a non-invasive diagnostic tool for postoperative follow-up of patients with mucinous ovarian cancer. |
Databáze: | OpenAIRE |
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