REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum Biomarker

Autor: Liisa Hattara, Laura Lehtinen, Kristiina Iljin, Pia Roering, Seija Grénman, John Patrick Mpindi, Taina Korpela, Annika Auranen, Katja Kaipio, Johanna Hynninen, Pia Vesterkvist, Caj Haglund, Harri Siitari, Olli Carpén, Ben Davidson
Přispěvatelé: Institute for Molecular Medicine Finland, Olli-Pekka Kallioniemi / Principal Investigator, Clinicum, Translational Cancer Biology (TCB) Research Programme, Department of Surgery, II kirurgian klinikka, Research Programs Unit, Precision Cancer Pathology
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Candidate gene
Pathology
Databases
Factual
endocrine system diseases
INVASION
Cancer Treatment
lcsh:Medicine
Gene Expression
Pancreatitis-Associated Proteins
Biochemistry
INTESTINAL-TYPE
Ovarian tumor
0302 clinical medicine
3123 Gynaecology and paediatrics
Medicine and Health Sciences
lcsh:Science
Ovarian Neoplasms
Multidisciplinary
Ascites
Adenocarcinoma
Mucinous
TUMORS
female genital diseases and pregnancy complications
3. Good health
Ovarian Cancer
REG4
ovarian cancer
Oncology
030220 oncology & carcinogenesis
Adenocarcinoma
Biomarker (medicine)
Immunohistochemistry
biomarker
Female
Endometrial Carcinoma
Research Article
medicine.medical_specialty
TISSUES
CARCINOMA
3122 Cancers
Gastroenterology and Hepatology
Biology
ta3111
Carcinomas
03 medical and health sciences
WAP Four-Disulfide Core Domain Protein 2
SDG 3 - Good Health and Well-being
medicine
Carcinoma
Biomarkers
Tumor
Cancer Detection and Diagnosis
Genetics
Humans
Lectins
C-Type
lcsh:R
ta1182
Proteins
Cancers and Neoplasms
Biology and Life Sciences
medicine.disease
ta3122
ta3123
PATTERN
030104 developmental biology
IV
CA-125 Antigen
lcsh:Q
3111 Biomedicine
Ovarian cancer
Gynecological Tumors
Biomarkers
Zdroj: Lehtinen, L, Vesterkvist, P, Roering, P, Korpela, T, Hattara, L, Kaipio, K, Mpindi, J-P, Hynninen, J, Auranen, A, Davidson, B, Haglund, C, Iljin, K, Grenman, S, Siitari, H & Carpen, O 2016, ' REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum Biomarker ', PLoS ONE, vol. 11, no. 3, e0151590 . https://doi.org/10.1371/journal.pone.0151590
PLoS ONE
PLoS ONE, Vol 11, Iss 3, p e0151590 (2016)
Popis: Preoperative diagnostics of ovarian neoplasms rely on ultrasound imaging and the serum biomarkers CA125 and HE4. However, these markers may be elevated in non-neoplastic conditions and may fail to identify most non-serous epithelial cancer subtypes. The objective of this study was to identify histotype-specific serum biomarkers for mucinous ovarian cancer. The candidate genes with mucinous histotype specific expression profile were identified from publicly available gene-expression databases and further in silico data mining was performed utilizing the MediSapiens database. Candidate biomarker validation was done using qRT-PCR, western blotting and immunohistochemical staining of tumor tissue microarrays. The expression level of the candidate gene in serum was compared to the serum CA125 and HE4 levels in a patient cohort of prospectively collected advanced ovarian cancer. Database searches identified REG4 as a potential biomarker with specificity for the mucinous ovarian cancer subtype. The specific expression within epithelial ovarian tumors was further confirmed by mRNA analysis. Immunohistochemical staining of ovarian tumor tissue arrays showed distinctive cytoplasmic expression pattern only in mucinous carcinomas and suggested differential expression between benign and malignant mucinous neoplasms. Finally, an ELISA based serum biomarker assay demonstrated increased expression only in patients with mucinous ovarian cancer. This study identifies REG4 as a potential serum biomarker for histotype-specific detection of mucinous ovarian cancer and suggests serum REG4 measurement as a non-invasive diagnostic tool for postoperative follow-up of patients with mucinous ovarian cancer.
Databáze: OpenAIRE
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