Circulating exosomes deliver free fatty acids from the bloodstream to cardiac cells: Possible role of CD36

Autor: Rafael S. Sánchez, Peter J. Simons, Aase Handberg, Nahuel A. García, José-Enrique O'Connor, Hernán González-King, Beatriz Jávega, Pilar Sepúlveda, Alicia Martínez-Romero, E. Grueso
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
CD36 Antigens
Male
Luminescence
CD36
Mice
SCID
Fatty Acids
Nonesterified
Exosomes
Biochemistry
Fats
Mice
Spectrum Analysis Techniques
Animal Cells
Mice
Inbred NOD
Medicine and Health Sciences
Myocytes
Cardiac
Tissue homeostasis
chemistry.chemical_classification
Cardiomyocytes
Multidisciplinary
biology
medicine.diagnostic_test
Physics
Electromagnetic Radiation
Fatty Acids
Heart
Flow Cytometry
Lipids
Cell biology
Blot
Spectrophotometry
Physical Sciences
Medicine
lipids (amino acids
peptides
and proteins)
Female
Cytophotometry
Cellular Structures and Organelles
Anatomy
Cellular Types
Research Article
Adult
Science
Muscle Tissue
Research and Analysis Methods
Fluorescence
Flow cytometry
Cell Line
03 medical and health sciences
In vivo
medicine
Diabetes Mellitus
Animals
Humans
Vesicles
Obesity
Rats
Wistar
Muscle Cells
030102 biochemistry & molecular biology
Fatty acid
Biology and Life Sciences
Cell Biology
Atherosclerosis
Microvesicles
Disease Models
Animal
030104 developmental biology
Biological Tissue
chemistry
biology.protein
Cardiovascular Anatomy
Ex vivo
Zdroj: PLoS ONE
Garcia, N A, González-King, H, Grueso, E, Sánchez, R, Martinez-Romero, A, Jávega, B, O'Connor, J E, Simons, P J, Handberg, A & Sepúlveda, P 2019, ' Circulating exosomes deliver free fatty acids from the bloodstream to cardiac cells : Possible role of CD36 ', PLOS ONE, vol. 14, no. 5, e0217546 . https://doi.org/10.1371/journal.pone.0217546
PLoS ONE, Vol 14, Iss 5, p e0217546 (2019)
Plos One
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
instname
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
ISSN: 1932-6203
Popis: Regulation of circulating free fatty acid (FFA) levels and delivery is crucial to maintain tissue homeostasis. Exosomes are nanomembranous vesicles that are released from diverse cell types and mediate intercellular communication by delivering bioactive molecules. Here, we sought to investigate the uptake of FFAs by circulating exosomes, the delivery of FFA-loaded exosomes to cardiac cells and the possible role of the FFA transporter CD36 in these processes. Circulating exosomes were purified from the serum of healthy donors after an overnight fast (F) or 20 minutes after a high caloric breakfast (postprandial, PP). Western blotting, Immunogold Electron Microscopy and FACS analysis of circulating exosomes showed that CD36 was expressed under both states, but was higher in postprandial-derived exosomes. Flow cytometry analysis showed that circulating exosomes were able to take-up FFA directly from serum. Importantly, preincubation of exosomes with a blocking CD36 antibody significantly impeded uptake of the FFA analogue BODIPY, pointing to the role of CD36 in FFA exosomal uptake. Finally, we found that circulating exosomes could delivery FFA analogue BODIPY into cardiac cells ex vivo and in vivo in a mice model. Overall, our results suggest a novel mechanism in which circulating exosomes can delivery FFAs from the bloodstream to cardiac tissue. Further studies will be necessary to understand this mechanism and, in particular, its potential involvement in metabolic pathologies such as obesity, diabetes and atherosclerosis.
Databáze: OpenAIRE
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