Improving Osteoblast Response In Vitro by a Nanostructured Thin Film with Titanium Carbide and Titanium Oxides Clustered around Graphitic Carbon

Autor: Francesco Mura, Giovanni Longo, Anna Scotto d'Abusco, Roberto Scandurra, Carlo Misiano, Luca Mazzola, Laura Politi, Fabiana Superti, Robertino Zanoni, Caterina Alexandra Ioannidu, Francesca Iosi
Jazyk: angličtina
Rok vydání: 2016
Předmět:
biomedical applications
Talin
0301 basic medicine
Integrins
Scanning electron microscope
lcsh:Medicine
02 engineering and technology
surface free-energy
chemistry.chemical_compound
Coated Materials
Biocompatible
Animal Cells
Medicine and Health Sciences
Electron Microscopy
Graphite
lcsh:Science
Free Energy
Connective Tissue Cells
Titanium
Thin Films
Microscopy
Multidisciplinary
Titanium carbide
Physics
respiratory system
021001 nanoscience & nanotechnology
Extracellular Matrix
Laboratory Equipment
modulation
Chemistry
integrin activation
Connective Tissue
Physical Sciences
Engineering and Technology
Thermodynamics
Scanning Electron Microscopy
Cellular Types
Anatomy
Cellular Structures and Organelles
0210 nano-technology
Layer (electronics)
Research Article
Chemical Elements
Materials science
Biocompatibility
Materials by Structure
proliferation
Materials Science
Equipment
chemistry.chemical_element
cell-adhesion
force spectroscopy
Research and Analysis Methods
03 medical and health sciences
Osseointegration
Coatings
Cell Line
Tumor
Cell Adhesion
Humans
bone-cells
extracellular-matrix
osseointegration
Thin film
Materials by Attribute
Cell Proliferation
Osteoblasts
Surface Treatments
Ion plating
lcsh:R
Integrin alpha3beta1
technology
industry
and agriculture
Biology and Life Sciences
Membranes
Artificial
Cell Biology
Laboratory Glassware
equipment and supplies
Nanostructures
Biological Tissue
030104 developmental biology
Manufacturing Processes
chemistry
Chemical engineering
lcsh:Q
Osteoblast Response In Vitro
Paxillin
Zdroj: PLoS ONE, Vol 11, Iss 3, p e0152566 (2016)
PLoS ONE
PloS one 11 (2016): e0152566. doi:10.1371/journal.pone.0152566
info:cnr-pdr/source/autori:Longo G.; Ioannidu C.A.; D'Abusco A.S.; Superti F.; Misiano C.; Zanoni R.; Politi L.; Mazzola L.; Iosi F.; Mura F.; Scandurra R./titolo:Improving osteoblast response in vitro by a nanostructured thin film with titanium carbide and titanium oxides clustered around graphitic carbon/doi:10.1371%2Fjournal.pone.0152566/rivista:PloS one/anno:2016/pagina_da:e0152566/pagina_a:/intervallo_pagine:e0152566/volume:11
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0152566
Popis: Introduction Recently, we introduced a new deposition method, based on Ion Plating Plasma Assisted technology, to coat titanium implants with a thin but hard nanostructured layer composed of titanium carbide and titanium oxides, clustered around graphitic carbon. The nanostructured layer has a double effect: protects the bulk titanium against the harsh conditions of biological tissues and in the same time has a stimulating action on osteoblasts. Results The aim of this work is to describe the biological effects of this layer on osteoblasts cultured in vitro. We demonstrate that the nanostructured layer causes an overexpression of many early genes correlated to proteins involved in bone turnover and an increase in the number of surface receptors for alpha 3 beta 1 integrin, talin, paxillin. Analyses at single-cell level, by scanning electron microscopy, atomic force microscopy, and single cell force spectroscopy, show how the proliferation, adhesion and spreading of cells cultured on coated titanium samples are higher than on uncoated titanium ones. Finally, the chemistry of the layer induces a better formation of blood clots and a higher number of adhered platelets, compared to the uncoated cases, and these are useful features to improve the speed of implant osseointegration. Conclusion In summary, the nanostructured TiC film, due to its physical and chemical properties, can be used to protect the implants and to improve their acceptance by the bone.
Databáze: OpenAIRE
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